葡萄膜黑色素瘤是一种眼部恶性肿瘤，其预后在转移后严重恶化。为了提高对转移性葡萄膜黑色素瘤分子生理学的理解，我们鉴定了与转移性和非转移性葡萄膜黑色素瘤相关的基因和通路。之前发布的基因表达综合数据集（GEO）用于识别转移性和非转移性葡萄膜黑色素瘤之间的差异表达基因。以及使用基因集富集分析 (GSEA)、EnrichR 和 iLINCS 进行通路和扰动分析。在男性转移性葡萄膜黑色素瘤样本中，基因 LOC401052 显着下调，FHDC1 显着上调。到非转移性男性样本。在女性样本中，没有发现显着差异表达的基因。此外，我们在男性转移性葡萄膜黑色素瘤中发现了许多显着上调的免疫反应途径，包括“免疫反应中的 T 细胞激活”。相比之下，许多上调的女性途径涉及铁代谢，包括“血红素生物合成过程”。 iLINCS 扰动因素分析发现，男性和女性样本与生长因子受体具有相似的不一致活性，但只有女性样本与孕酮受体激动剂具有不一致的活性。我们的基因、通路和扰动因素分析结果表明，性别之间转移过程存在差异。版权© 2024，国际抗癌研究所（George J. Delinasios 博士），保留所有权利。

Uveal melanoma is an ocular malignancy whose prognosis severely worsens following metastasis. In order to improve the understanding of molecular physiology of metastatic uveal melanoma, we identified genes and pathways implicated in metastatic vs non-metastatic uveal melanoma.A previously published dataset from Gene Expression Omnibus (GEO) was used to identify differentially expressed genes between metastatic and non-metastatic samples as well as to conduct pathway and perturbagen analyses using Gene Set Enrichment Analysis (GSEA), EnrichR, and iLINCS.In male metastatic uveal melanoma samples, the gene LOC401052 is significantly down-regulated and FHDC1 is significantly up-regulated compared to non-metastatic male samples. In female samples, no significant differently expressed genes were found. Additionally, we identified many significant up-regulated immune response pathways in male metastatic uveal melanoma, including "T cell activation in immune response". In contrast, many top up-regulated female pathways involve iron metabolism, including "heme biosynthetic process". iLINCS perturbagen analysis identified that both male and female samples have similar discordant activity with growth factor receptors, but only female samples have discordant activity with progesterone receptor agonists.Our results from analyzing genes, pathways, and perturbagens demonstrate differences in metastatic processes between sexes.Copyright © 2024, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.