乳腺癌（BC）是全世界女性最常见的肿瘤。 TRIM28 (RNF96) 发挥多效性生物学功能，例如沉默靶基因、促进 DNA 修复、刺激细胞增殖和分化以及促进癌症进展。 TRIM28 在癌症中发挥着越来越重要的作用，但其对 BC（包括乳腺浸润性癌）的影响仍知之甚少。在当前的研究中，对乳腺浸润性癌（BRCA）患者进行了在线数据库、实时定量PCR、免疫组织化学和蛋白质印迹分析。虫草素 (CD) 用于监测 BC 进展和体内 TRIM28 表达。结果，我们观察到与相应的正常组织相比，TRIM28在乳腺浸润性癌组织中高表达，并且与转移/浸润进展相关。 TRIM28 的高表达可能作为三阴性 BC、晚期 BC 或乳腺浸润性癌长期生存的预后标志物。虽然乳腺浸润性癌肿瘤组织中TRIM28甲基化与匹配的正常组织相比没有显着变化，但TRIM28的表达与甲基化呈显着负相关。在小鼠模型中，TRIM28 的表达被 CD 抑制，表明其在预防 BC 进展中的作用。因此，TRIM28可能是预测乳腺浸润性癌患者进展/预后的潜在有价值的分子靶点。 CD 可抑制 BC 生长/转移，可能部分通过抑制 TRIM28 表达来参与。© 作者。

Breast cancer (BC) is the most common tumor in women worldwide. TRIM28 (RNF96) plays pleiotropic biological functions, such as silencing target genes, facilitating DNA repair, stimulating cellular proliferation and differentiation, and contributing to cancer progression. TRIM28 plays an increasingly crucial role in cancer, but its impact on BC, including breast invasive carcinoma, remains poorly understood. In the current study, analyses of online databases, quantitative real-time quantitative PCR, immunohistochemistry, and western blotting were performed on patients with breast invasive carcinoma (BRCA). Cordycepin (CD) was used to monitor BC progression and TRIM28 expression in vivo. As a result, we observed that TRIM28 is highly expressed in breast invasive carcinoma tissues compared with the corresponding normal tissues and is correlated with metastatic / invasive progression. High expression of TRIM28 might serve as a prognostic marker for long-term survival in triple-negative BC, advanced BC, or breast invasive carcinoma. Although TRIM28 methylation in tumor tissues of breast invasive carcinoma is not significantly changed compared to the matched normal tissues, the expressions and methylation of TRIM28 are significantly reversely correlated. TRIM28 expression was inhibited by CD in the mouse model, indicating its role in preventing BC progression. Thus, TRIM28 might be a potentially valuable molecular target for forecasting the progression / prognosis of patients with breast invasive carcinoma. CD, which represses BC growth/metastasis, may be involved partially through suppressing TRIM28 expression.© The author(s).