在本研究中，使用印楝叶水提取物合成氧化锌纳米粒子（ZnO-NP），并使用透射电子显微镜（TEM）、紫外可见光谱（UV-Vis）和动态光散射（DLS）进行表征。然后将其与化学合成的 ZnO-NPs 和用于绿色合成 ZnO-NPs 的印楝叶提取物的抗癌和抗菌功效进行比较。 TEM、UV-vis 和粒径证实所开发的 ZnO-NPs 是纳米级的。化学合成和绿色合成的ZnO-NP分别在328 nm和380 nm处显示出吸光度，并且观察到尺寸分别约为85 nm和62.5 nm的球形颗粒。利用 HPLC 和 GC-MS 鉴定了用于环保生产 ZnO-NP 的印楝叶水提取物中的生物活性成分。 HPLC分析表明，印楝叶水提取物含有19种酚类成分。 GC-MS 分析显示存在 21 种生物活性化合物。观察到不同浓度 (31.25 µg/mL-1000 µg/mL) 的绿色 ZnO-NPs 对 Hct 116 和 A 549 癌细胞的抗增殖作用，A 549 和 118 µg/mL 的 IC50 值为 111 µg/mL Hct 116。另一方面，估计了针对革兰氏阳性菌和革兰氏阴性菌的抗菌活性。抗菌结果表明，绿色合成的ZnO-NPs对革兰氏阳性菌和革兰氏阴性菌的MIC分别为5、1μg/mL。因此，它们可以用作有效的抗菌和抗增殖剂。©2024 El-Beltagi 等人。

In the present study, zinc oxide nanoparticles (ZnO-NPs) were synthesized using neem leaf aqueous extracts and characterized using transmission electron microscopy (TEM), ultraviolet visible spectroscopy (UV-Vis), and dynamic light scattering (DLS). Then compare its efficacy as anticancer and antibacterial agents with chemically synthesized ZnO-NPs and the neem leaf extract used for the green synthesis of ZnO-NPs. The TEM, UV-vis, and particle size confirmed that the developed ZnO-NPs are nanoscale. The chemically and greenly synthesized ZnO-NPs showed their optical absorbance at 328 nm and 380 nm, respectively, and were observed as spherical particles with a size of about 85 nm and 62.5 nm, respectively. HPLC and GC-MS were utilized to identify the bioactive components in the neem leaf aqueous extract employed for the eco-friendly production of ZnO-NPs. The HPLC analysis revealed that the aqueous extract of neem leaf contains 19 phenolic component fractions. The GC-MS analysis revealed the existence of 21 bioactive compounds. The antiproliferative effect of green ZnO-NPs was observed at different concentrations (31.25 µg/mL-1000 µg/mL) on Hct 116 and A 549 cancer cells, with an IC50 value of 111 µg/mL for A 549 and 118 µg/mL for Hct 116. On the other hand, the antibacterial activity against gram-positive and gram-negative bacteria was estimated. The antibacterial result showed that the MIC of green synthesized ZnO-NPs against gram-positive and gram-negative bacteria were 5, and 1 µg/mL. Hence, they could be utilized as effective antibacterial and antiproliferative agents.©2024 El-Beltagi et al.