本研究旨在探讨吴茱萸碱对银屑病和银屑病瘙痒的作用。以咪喹莫特诱导的小鼠银屑病样皮炎为模型，局部应用吴茱萸碱7 d。每天观察小鼠背部皮肤损伤、临床评分并记录它们的抓挠行为。实验最后一天采集血样，采用酶联免疫吸附法测定血清中瘙痒相关炎症细胞因子肿瘤坏死因子（TNF）-α、白细胞介素（IL）-23、IL-17A的水平。 。在苏木精和伊红染色的皮肤标本中观察到组织病理学变化。皮损中瞬时受体电位香草酸（TRPV）1、TRPV3、TRPV4以及瘙痒相关介质P物质（SP）、神经生长因子（NGF）、降钙素基因相关肽（CGRP）的表达水平使用蛋白质印迹和 qRT-PCR 进行分析。使用旷场、悬浮和旋转棒实验评估吴茱萸碱对小鼠探索行为、运动和协调能力的影响。利用分子对接验证吴茱萸碱与TRPV1、TRPV3、TRPV4残基的结合。吴茱萸碱通过降低血清中炎症介质TNF-α、IL-23、IL-17A的水平来减轻瘙痒、抑制炎症。咪喹莫特诱导小鼠皮肤中 SP、NGF、CGRP、TRPV1、TRPV3 和 TRPV4 的 mRNA 和蛋白表达水平减弱。吴茱萸碱由于其抑制免疫炎症和瘙痒介质的能力，可有效治疗牛皮癣和瘙痒症.© 2024 梁等人。

This study seeks to investigate the effect of evodiamine on psoriasis and psoriatic pruritus.Imiquimod-induced psoriasiform dermatitis in mice was used as a model, and evodiamine was topically applied for seven days. The mice were observed daily for skin damage on the back, clinical score and their scratching behavior was recorded. Blood samples were collected on the final day of the experiment, and the serum levels of pruritus-associated inflammatory cytokines tumor necrosis factor (TNF) -α, interleukin (IL) -23, and IL-17A were measured using enzyme-linked immunosorbent assay. Histopathological changes were observed in Hematoxylin and Eosin-stained skin specimens. The expression levels of transient receptor potential vanilloid (TRPV) 1, TRPV3, TRPV4, and the pruritus-related mediators Substance P (SP), nerve growth factor (NGF), and calcitonin gene-related peptide (CGRP) in the skin lesions were analyzed using Western blot and qRT-PCR. The effect of evodiamine on the exploratory behavior, motor, and coordination abilities of mice was assessed using open field, suspension, and Rota-Rod experiments. Molecular docking was utilized to verify the binding of evodiamine to the residues of TRPV1, TRPV3, and TRPV4.Evodiamine reduced pruritus and inhibited inflammation by decreasing the levels of inflammatory mediators TNF-α, IL-23, and IL-17A in the serum of Imiquimod-induced mice and attenuated the mRNA and protein expression levels of SP, NGF, CGRP, TRPV1, TRPV3, and TRPV4 in the skin.Evodiamine is an effective treatment for psoriasis and pruritus, due to its ability to inhibit immune inflammation and pruritic mediators.© 2024 Liang et al.