光动力疗法（PDT）是一种用于治疗皮肤癌和其他非感染性疾病的既定疗法。最近人们对使用 aPDT（抗菌 PDT）治疗皮肤和软组织感染的机会产生了兴趣。 PDT 利用光敏剂渗透所有细胞并使它们对给定波长的光“敏感”。光敏剂对给定波长的光具有高度吸收性，并且在有氧存在的情况下被激发时会产生破坏性氧自由基和单线态氧。与人类细胞相比，细菌细胞对抗氧化应激的能力相对较差，因此 aPDT 可以实现一定程度的选择性毒性。在本章中，我们概述了使用标准实验室设备在体外测试 aPDT 的方法。© 2024。 ），获得 Springer Science Business Media, LLC（Springer Nature 的一部分）的独家许可。

Photodynamic therapy (PDT) is an established therapy used for the treatment of cutaneous skin cancers and other non-infective ailments. There has been recent interest in the opportunity to use aPDT (antimicrobial PDT) to treat skin and soft tissue infections. PDT utilizes photosensitizers that infiltrate all cells and "sensitize" them to a given wavelength of light. The photosensitizer is simply highly absorbent to a given wavelength of light and when excited will produce, in the presence of oxygen, damaging oxygen radicals and singlet oxygen. Bacterial cells are comparatively poor at combatting oxidative stress when compared with human cells therefore a degree of selective toxicity can be achieved with aPDT.In this chapter, we outline methodologies for testing aPDT in vitro using standard lab equipment.© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.