土耳其榛子（Corylus avellana L. cv Tombul）是巧克力行业中广泛使用的坚果，还富含多酚含量，具有抗癌作用。检查榛子叶提取物对肺癌和乳腺癌细胞的抗癌和细胞凋亡作用。磺胺罗丹明 B (SRB) 和 5'-三磷酸腺苷 (ATP) 测定用于细胞活力测量。通过双荧光染色在形态学上显示细胞死亡的模式。通过进行 caspase 介导的细胞角蛋白 18 (M30 ELISA) 和蛋白质印迹分析来确定细胞凋亡。 PARP、caspase 3、caspase 8、DR4 和 GAPHD（3-磷酸甘油醛脱氢酶）蛋白条带可视化为细胞凋亡标记。采用伤口愈合测试来测量细胞迁移。榛子叶的甲醇提取物以剂量依赖性方式表现出对细胞生长活性的抑制作用。对于乙醇提取物，MCF-7 中的 IC50 值为 32.17 µg/ml，MDA-MB-231 中为 32.16 µg/ml，A549 中为 20.40 µg/ml，H1299 细胞中为 12.04 µg/ml，而其测定值为 21.08 µg/ml。甲醇提取物中 MCF-7 细胞中浓度为 40.16 µg/ml，MDA-MB-231 中浓度为 40.16 µg/ml，A549 细胞中浓度为 22.04 µg/ml，H1299 细胞中浓度为 5.91 µg/ml。相比之下，甲醇叶提取物对 H1299 细胞更有效（IC50 值为 5.91 µg/ml）。相比之下，乙醇叶提取物对 H1299 细胞更有效（IC50 值为 9.722 µg/ml）。蛋白质印迹分析表明，榛子叶提取物处理癌细胞导致细胞通过凋亡死亡，并抑制肺癌和乳腺癌细胞系中的细胞迁移。榛子提取物对乳腺癌和肺癌细胞的细胞毒性作用对于阐明细胞死亡机制以开发癌症治疗新方法可能具有价值和前景。© 2024。作者。

Turkish hazelnut (Corylus avellana L. cv Tombul) is a widely used nut in the chocolate industry and is also rich in polyphenol content, which promises anticancer effects. The anti-cancer and apoptotic effects of hazelnut leaves extracts examined on lung and breast cancer cells. Sulforhodamine B (SRB) and Adenosine 5'- triphosphate (ATP) assays were carried out for cell viability measurement. The mode of cell death was shown morphologically by the double fluorescence staining. Apoptosis was determined by performing caspase-mediated cytokeratin 18 (M30 ELISA) and western blot analysis. PARP, caspase 3, caspase 8, DR4, and GAPHD (Glyceraldehyde-3-phosphate Dehydrogenase) protein bands were visualized as markers of apoptosis. A wound healing test was employed to measure cell migration. Methanol extract of hazelnut leaf exhibited inhibition of cell growth activities in a dose-dependent manner. IC50 values were determined as 32.17 µg/ml in MCF-7, 32.16 µg/ml in MDA-MB-231, 20.40 µg/ml in A549 and 12.04 µg/ml in H1299 cells for ethanol extract while it was determined as 21.08 µg/ml in MCF-7, 40.16 µg/ml in MDA-MB-231, 22.04 µg/ml in A549 and 5.91 µg/ml in H1299 cells in methanol extract. In comparison, methanol leaf extracts were more effective in H1299 cells (IC50 value was 5.91 µg/ml).In comparison, ethanol leaf extracts were more effective in H1299 cells (IC50 value was 9.722 µg/ml). Western blot analysis demonstrated that hazelnut leaf extract treatment of cancer cells led to cell death via apoptosis and inhibited cell migration in lung and breast cancer cell lines. The cytotoxic effects of hazelnut extract on breast and lung cancer cells might be valuable and promising in elucidating cell death mechanisms for the development of new methods in cancer treatment.© 2024. The Author(s).