黑色素瘤是一种侵袭性皮肤癌，具有较高的转移倾向。肿瘤患者治疗中的主要关键问题之一是现有疗法的毒性和耐药性的发展。纳米技术领域已经取得了巨大进展，以限制抗癌治疗的不良影响。我们探索了创建由油酸组成的水包油纳米乳液作为亲脂性药物递送的生物活性载体的潜力。这种生物活性纳米乳剂负载有姜黄素（一种天然荧光亲脂性化合物），用作模型药物来评估纳米乳剂的以下能力：i) 封装亲脂性部分； ii) 与特定细胞相互作用，以及 iii) 与游离药物相比，提高了负载模型药物的功效。因此，我们评估了姜黄素纳米乳液的物理化学特征，确认其 pH 敏感性及其随时间的稳定性。此外，纳米乳液能够通过降解/不稳定现象保存负载的姜黄素。最后，我们在 B16F10 黑色素瘤细胞系中验证了纳米乳剂传递的姜黄素的一些生物学功能。我们获得了姜黄素生物作用的证据，表明基于油酸的纳米乳液可作为亲脂性药物输送的有效纳米载体。版权所有 © 2024。由 Elsevier B.V. 出版。

Melanoma is an aggressive form of skin cancer with elevated propensity to metastasize. One of the major critical issues in the treatment of oncological patients is represented by the development of toxicity and resistance to the available therapies. Great progress has been made in the field of nanotechnologies to limit the unwanted effects of anti-cancer treatments. We explored the potential of creating oil-in-water nanoemulsions composed of oleic acid, as a bioactive carrier for lipophilic drug delivery. This bioactive nanoemulsion was loaded with Curcumin, a natural fluorescent lipophilic compound, used as a model drug to evaluate nanoemulsion capability to: i) encapsulate the lipophilic moiety; ii) interact with the specific cells, and iii) improve the efficacy of the loaded model drug compared to the free one. Therefore, we evaluated the physical-chemical features of Curcumin-loaded nanoemulsions, confirming their pH sensibility and their stability over time. Moreover, the nanoemulsions were able to preserve the loaded Curcumin by degradation/destabilization phenomena. Finally, we verified some of the biological functions of Curcumin delivered by nanoemulsions in the B16F10 melanoma cell line. We obtained evidence of the biological action of Curcumin, suggesting oleic-based nanoemulsions as an efficient nanocarrier for lipophilic drug delivery.Copyright © 2024. Published by Elsevier B.V.