癌症和糖尿病等慢性病是印度和全世界的主要公共卫生问题。如今，治疗这些疾病对植物源产品的需求很大。南瓜子传统上因其药理特性而受到关注，良性前列腺增生就是例证。此前，人们通过奥斯本法提取了南瓜籽蛋白，并对其功能和营养品质进行了评价。此处的目的是在体外评估种子蛋白组分的抗癌和抗糖尿病特性。 HepG2、MDA-MB-231 和 MCF-7 细胞系用水溶性 (WF) 和碱溶性组分 (AF) 处理以评估细胞毒性，而胰腺 β 细胞和胰岛素抵抗 (IR) - HepG2 细胞系接受 WF 治疗以评估其抗糖尿病潜力。 WF 和 AF 对 HepG2 和 MDA-MB-231 细胞系表现出细胞毒性作用，表明细胞凋亡介导的抗癌活性。 WF 以剂量依赖性方式增强胰腺 β 细胞中葡萄糖刺激的胰岛素分泌。在 IR-HepG2 细胞系研究中，与糖尿病组相比，对照组、二甲双胍和 WF 治疗组显示出葡萄糖的摄取，这与这些组中 GLUT2 和 GLUT4 转运蛋白的表达上调密切相关。这些结果表明，来自 WF 和 AF 的蛋白质可能具有抗癌和抗糖尿病特性，因此有可能利用南瓜蛋白来治疗癌症和糖尿病。© 2024。作者获得 Springer Science Business Media 的独家许可， LLC，施普林格自然集团的一部分。

Chronic diseases like cancer and diabetes are the major public health concerns of India and worldwide. Nowadays, plant-derived products are in great demand for the treatment of these diseases. Pumpkin seeds are traditionally implicated for their pharmacological properties, as exemplified by benign prostatic hyperplasia. Earlier, pumpkin seed proteins were extracted by the Osborne method, and their functional and nutritional qualities were evaluated. Here, the aim is to assess in vitro, the anticancer and antidiabetic properties of seed protein fractions. HepG2, MDA-MB-231, and MCF-7 cell lines were treated with water-soluble (WF) and alkali-soluble fractions (AF) to assess cytotoxicity, while pancreatic β-cells and insulin resistance (IR) - HepG2 cell lines were treated with WF to evaluate the antidiabetic potential. WF and AF showed cytotoxic effects towards HepG2 and MDA-MB-231 cell lines, suggesting apoptosis-mediated anticancerous activity. WF potentiates glucose-stimulated insulin secretion in pancreatic β-cells, in a dose-dependent manner. In IR-HepG2 cell line studies, control, metformin, and WF-treated groups showed uptake of glucose, when compared to the diabetic group, which is well-correlated with the upregulated expressions of GLUT2 and GLUT4 transporters in these groups. These results indicate that proteins from WF and AF may have anticancerous and antidiabetic properties and thus have the potential to utilize pumpkin proteins in the management of cancer and diabetes.© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.