肿瘤浸润免疫细胞 (TIIC) 的空间背景对于预测结直肠癌 (CRC) 患者的临床结果非常重要。然而，TIIC 空间分布的预后价值尚不清楚。因此，我们的目的是在大量 CRC 样本中研究原位 TIIC 与患者预后之间的关联。我们在 190 个 CRC 样本中实施多重免疫组织化学染色技术，以原位量化 14 个 TIIC 亚组。为了描绘TIIC与肿瘤细胞的空间关系，基于图像识别技术将组织切片分割为肿瘤细胞和微环境区室，并通过执行计算管道phenoptr计算免疫细胞和肿瘤细胞之间的距离。MPO中性粒细胞和CD68 IDO1肿瘤相关巨噬细胞（TAM）在上皮区室中富集，骨髓谱系细胞距离肿瘤细胞最近。除CD68 CD163 TAM外，其他细胞均与良好预后呈正相关。 TIIC 的预后预测能力与其与肿瘤细胞的距离高度相关。无监督聚类分析将结直肠癌分为具有不同预后结果的三种亚型，相关性分析揭示了 B 细胞、CD68 IDO1 TAM 和 T 谱系细胞在产生有效免疫反应方面的协同作用。我们的研究表明，空间定位与 TIIC 的整合丰度对于综合预后评估很重要。© 2024。作者。

The spatial context of tumor-infiltrating immune cells (TIICs) is important in predicting colorectal cancer (CRC) patients' clinical outcomes. However, the prognostic value of the TIIC spatial distribution is unknown. Thus, we aimed to investigate the association between TIICs in situ and patient prognosis in a large CRC sample.We implemented multiplex immunohistochemistry staining technology in 190 CRC samples to quantify 14 TIIC subgroups in situ. To delineate the spatial relationship of TIICs to tumor cells, tissue slides were segmented into tumor cell and microenvironment compartments based on image recognition technology, and the distance between immune and tumor cells was calculated by implementing the computational pipeline phenoptr.MPO+ neutrophils and CD68+IDO1+ tumor-associated macrophages (TAMs) were enriched in the epithelial compartment, and myeloid lineage cells were located nearest to tumor cells. Except for CD68+CD163+ TAMs, other cells were all positively associated with favorable prognosis. The prognostic predictive power of TIICs was highly related to their distance to tumor cells. Unsupervised clustering analysis divided colorectal cancer into three subtypes with distinct prognostic outcomes, and correlation analysis revealed the synergy among B cells, CD68+IDO1+TAMs, and T lineage cells in producing an effective immune response.Our study suggests that the integration of spatial localization with TIIC abundance is important for comprehensive prognostic assessment.© 2024. The Author(s).