受体蛋白酪氨酸磷酸酶 γ 和 ζ（RPTPγ 和 RPTPζ）是具有胞外碳酸酐酶样结构域的跨膜信号蛋白，在中枢神经系统 (CNS) 的发育和功能中发挥重要作用，并与肿瘤抑制、神经退行性变和感知细胞外[CO2]和[HCO3 -]。 RPTPγ 在全身表达，而 RPTPδ 优先在 CNS 中表达。在这里，我们研究了来自 C57BL/6 野生型实验室品系小鼠的三个来源中 RPTPγ-RPTPδ 表达的差异：（a）从 P0-P2 幼崽分离后 14 天的混合神经元-星形胶质细胞海马（HC）培养物； (b) P0-P2 幼犬海马体； (c) 9 至 12 周大的成年海马体。关于 RPTPγ，我们检测到 Ptprg 变体 1 (V1) 转录本，代表典型的外显子 1-30。此外，我们新验证了假设的装配体 [XM_006517956]（建议名称，Ptprg-V3），它缺少外显子 14。两个转录本都存在于所有三个 HC 来源中。关于 RPTP，我们确认了 Ptprz1-V1 的表达，在幼犬和成年犬中检测到它，但在培养物中未检测到它，并在所有三种制剂中检测到 Ptprz1-V3 到 Ptprz1-V7。我们新验证了假设的组件 Ptprz1-X1（在培养物和幼犬中）、Ptprz1-X2（在所有三个中）和 Ptprz1-X5（在幼犬和成年犬中），并建议将它们重新指定为 Ptprz1-V0、Ptprz1-V2、和Ptprz1-V8，分别。 RPTPγ 和 RPTPζ 剪接变体的多样性可能对应于不同细胞区室、发育期间和以后生命期间的不同信号传导功能。与之前报道的在神经元和有时在神经胶质细胞中不同的 RPTPγ 和 RPTPδ 蛋白表达的研究相比，我们观察到在所有三种 HC 制剂中，RPTPγ 和 RPTPδ 在几乎所有 HC 神经元的体细胞和突起中共表达，但不在星形胶质细胞中表达.版权所有 © 2024 Taki、Boron 和 Moss。

Receptor protein tyrosine phosphatases γ and ζ (RPTPγ and RPTPζ) are transmembrane signaling proteins with extracellular carbonic anhydrase-like domains that play vital roles in the development and functioning of the central nervous system (CNS) and are implicated in tumor suppression, neurodegeneration, and sensing of extracellular [CO2] and [HCO3 -]. RPTPγ expresses throughout the body, whereas RPTPζ preferentially expresses in the CNS. Here, we investigate differential RPTPγ-RPTPζ expression in three sources derived from a wild-type laboratory strain of C57BL/6 mice: (a) mixed neuron-astrocyte hippocampal (HC) cultures 14 days post isolation from P0-P2 pups; (b) P0-P2 pup hippocampi; and (c) 9- to 12-week-old adult hippocampi. Regarding RPTPγ, we detect the Ptprg variant-1 (V1) transcript, representing canonical exons 1-30. Moreover, we newly validate the hypothetical assembly [XM_006517956] (propose name, Ptprg-V3), which lacks exon 14. Both transcripts are in all three HC sources. Regarding RPTPζ, we confirm the expression of Ptprz1-V1, detecting it in pups and adults but not in cultures, and Ptprz1-V3 through Ptprz1-V7 in all three preparations. We newly validate hypothetical assemblies Ptprz1-X1 (in cultures and pups), Ptprz1-X2 (in all three), and Ptprz1-X5 (in pups and adults) and propose to re-designate them as Ptprz1-V0, Ptprz1-V2, and Ptprz1-V8, respectively. The diversity of RPTPγ and RPTPζ splice variants likely corresponds to distinct signaling functions, in different cellular compartments, during development vs later life. In contrast to previous studies that report divergent RPTPγ and RPTPζ protein expressions in neurons and sometimes in the glia, we observe that RPTPγ and RPTPζ co-express in the somata and processes of almost all HC neurons but not in astrocytes, in all three HC preparations.Copyright © 2024 Taki, Boron and Moss.