当归是中医长期使用的药物，以其补血活血的功效而闻名。当归中的阿魏酸、藁本内酯、丁子香酚活血化瘀；但其补血作用的物质基础有待进一步研究。本研究分离了五种同质当归多糖，并测定了它们的糖含量、分子量、单糖组成和红外特征。以乙酰苯肼（APH）和环磷酰胺（CTX）为诱导剂建立小鼠血虚模型，并测定小鼠脏器指数、血液生化参数。体内造血活性结果表明，当归多糖（APS）可升高血清促红细胞生成素（EPO）、粒细胞集落刺激因子（G-CSF）和白介素-3（IL-3）水平，降低肿瘤坏死因子-α小鼠体内TNF-α水平，通过调节细胞因子水平促进体内造血。体外补血生物效价测试结果表明，APS-H2O的补血活性最强，APS-0.4的造血活性最弱。对APS-H2O和APS-0.4的结构进行了表征，结果表明APS-H2O是一种阿拉伯半乳聚糖，主链由α-1,3,5-Ara(f)、α-1,5-组成。 Ara(f)、β-1,4-Gal(p) 和 β-1,4-Gal(p)A 以及 β-t-Gal(p) 和 α-t-Glc(p) 的两条支链) 以 (1→3) 连接方式与主链上的 α-1,3,5-Ara(f) 相互连接。 APS-0.4是一种以半乳糖醛酸为主链的酸性多糖，由α-1,4-GalA、α-1,2-GalA、α-1,4-Gal和β-1,4-Rha组成。综上所述，APS-H2O可作为一种潜在的血虚患者补血药物，为APS在临床治疗和保健食品中的应用以及新型多糖类药物的研发提供依据。版权所有© 2024 田、沉、胡、梁、丁、戴、刘、卢、尹、舒、郭、苏、李。

Angelica sinensis is a long-standing medicine used by Chinese medical practitioners and well-known for its blood-tonic and blood-activating effects. Ferulic acid, ligustilide, and eugenol in Angelica sinensis activate the blood circulation; however, the material basis of their blood-tonic effects needs to be further investigated. In this study, five homogeneous Angelica sinensis polysaccharides were isolated, and their sugar content, molecular weight, monosaccharide composition, and infrared characteristics determined. Acetylphenylhydrazine (APH) and cyclophosphamide (CTX) were used as inducers to establish a blood deficiency model in mice, and organ indices, haematological and biochemical parameters were measured in mice. Results of in vivo hematopoietic activity showed that Angelica sinensis polysaccharide (APS) could elevate erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), and interleukin-3 (IL-3) serum levels, reduce tumor necrosis factor-α (TNF-α) level in mice, and promote hematopoiesis in the body by regulating cytokine levels. Biological potency test results of the in vitro blood supplementation indicated strongest tonic activity for APS-H2O, and APS-0.4 has the weakest haemopoietic activity. The structures of APS-H2O and APS-0.4 were characterized, and the results showed that APS-H2O is an arabinogalactan glycan with a main chain consisting of α-1,3,5-Ara(f), α-1,5- Ara(f), β-1,4-Gal(p), and β-1,4-Gal(p)A, and two branched chains of β-t-Gal(p) and α-t-Glc(p) connected to each other in a (1→3) linkage to α-1,3,5-Ara(f) on the main chain. APS-0.4 is an acidic polysaccharide with galacturonic acid as the main chain, consisting of α-1,4-GalA, α-1,2-GalA, α-1,4-Gal, and β-1,4-Rha. In conclusion, APS-H2O can be used as a potential drug for blood replenishment in patients with blood deficiency, providing a basis for APS application in clinical treatment and health foods, as well as research and development of new polysaccharide-based drugs.Copyright © 2024 Tian, Shen, Hu, Liang, Ding, Dai, Liu, Lu, Yin, Shu, Guo, Su and Li.