由于细胞因子、趋化因子、生长因子和可溶性受体在结直肠癌 (CRC) 的发病机制中发挥重要作用，本研究分析了 CRC 患者和无癌对照患者的血清谱，作为可能的 CRC 特征。使用 ProcartaPlex 人类免疫监测 65-Plex Panel 测量 CRC 患者以及年龄和性别匹配的无癌症对照患者的 65 种分析物的血清水平。在 65 种测试分析物中，8 种细胞因子（CSF-3、IFN-γ、IL-12p70、IL-18、IL-20、MIF、TNF-α 和 TSLP）、8 种趋化因子（fractalkine、MIP-1β、BLC、Eotaxin） -1、Eotaxin-2、IP-10、MIP-1a、MIP-3a)、2 种生长因子（FGF-2、MMP-1）和 4 种可溶性受体（APRIL、CD30、TNFRII 和 TWEAK）存在差异以CRC表示。 ROC 分析证实 TNF-α、BLC、Eotaxin-1、APRIL 和 Tweak 与 AUC > 0.70 高度相关，提示治疗诊断应用。与非转移性 CRC 相比，转移性 CRC 中 IFN-γ、IL-18、MIF、BLC、Eotaxin-1、Eotaxin-2、IP-10 和 MMP1 的表达较低；仅MIF和MIP-1β的AUC为≥0.7。此外，MDC、IL-7、MIF、IL-21 和 TNF-α 与 CRC 化疗 (CT) 耐受性呈正相关（AUC > 0.7），而 IL-31、Fractalkine、Eotaxin-1 和 Eotaxin-2与 CT 耐药呈正相关。 TNF-α、BLC、Eotaxin-1、APRIL 和 Tweak 可用作 CRC 的一线早期检测。转移性和非转移性病例之间 MIF 和 MIP-1β 水平的差异决定了这些因素在 CRC 进展中的预后性质。关于 CT 耐受性，当观察到下调或对治疗产生耐药性时，MDC、IL-7、MIF、IL-21 和 TNF-α 是关键。© 2024。作者。

Insofar as they play an important role in the pathogenesis of colorectal cancer (CRC), this study analyzes the serum profile of cytokines, chemokines, growth factors, and soluble receptors in patients with CRC and cancer-free controls as possible CRC signatures. Serum levels of 65 analytes were measured in patients with CRC and age- and sex-matched cancer-free controls using the ProcartaPlex Human Immune Monitoring 65-Plex Panel. Of the 65 tested analytes, 8 cytokines (CSF-3, IFN-γ, IL-12p70, IL-18, IL-20, MIF, TNF-α and TSLP), 8 chemokines (fractalkine, MIP-1β, BLC, Eotaxin-1, Eotaxin-2, IP-10, MIP-1a, MIP-3a), 2 growth factors (FGF-2, MMP-1), and 4 soluble receptors (APRIL, CD30, TNFRII, and TWEAK), were differentially expressed in CRC. ROC analysis confirmed the high association of TNF-α, BLC, Eotaxin-1, APRIL, and Tweak with AUC > 0.70, suggesting theranostic application. The expression of IFN-γ, IL-18, MIF, BLC, Eotaxin-1, Eotaxin-2, IP-10, and MMP1 was lower in metastatic compared to non-metastatic CRC; only AUC of MIF and MIP-1β were > 0.7. Moreover, MDC, IL-7, MIF, IL-21, and TNF-α are positively associated with tolerance to CRC chemotherapy (CT) (AUC > 0.7), whereas IL-31, Fractalkine, Eotaxin-1, and Eotaxin-2 were positively associated with resistance to CT. TNF-α, BLC, Eotaxin-1, APRIL, and Tweak may be used as first-line early detection of CRC. The variable levels of MIF and MIP-1β between metastatic and non-metastatic cases assign prognostic nature to these factors in CRC progression. Regarding tolerance to CT, MDC, IL-7, MIF, IL-21, and TNF-α are key when down-regulated or resistant to treatment is observed.© 2024. The Author(s).