研究人参miRNA156对免疫和能量代谢的跨界调控，为进一步探索人参miRNA156作为药效物质的可能性提供新的视角。结合我们课题组前期的研究成果，高表达的miRNA156在血液测序中选择了人参汤灌胃。对选定的差异miRNA156进行生物信息学分析。差异miRNA156的靶基因主要富集于代谢、免疫等信号通路。根据分析结果，实验部分将采用气虚疲劳模型和RAW264.7细胞。乳酸（LA）、肌酸激酶（CK）、血尿素氮（BUN）、乳酸脱氢酶（LD）、肝糖原（LG）、肌糖原（MG）、白细胞介素4（IL-4）、基质金属含量-蛋白酶9 (MMP-9)、超氧化物歧化酶(SOD)、丙二醛、磷酸烯醇丙酮酸羧激酶(PEPCK)、葡萄糖-6-磷酸酶(G6pase)、一氧化氮(NO)和肿瘤坏死因子-α(TNF-α)服用miRNA156后进行测量。人参miRNA156可以加速运动过程中代谢废物的清除。增加糖原储备，为机体提供能量，调节关键糖异生酶磷的活性，改善能量代谢系统，增强疲劳小鼠的耐力。基质金属蛋白酶9、超氧化物歧化酶、丙二醛的含量受到影响，RAW264.7细胞上清液中TNF-α的含量显着升高，具有一定的抗氧化能力和潜在的免疫调节作用。人参miRNA156具有一定的调节作用小鼠能量代谢和免疫功能的研究，使得理论上人参miRNA的跨物种调控成为可能，为人参miRNA成为新的药效物质提供思路。Copyright © 2024 Elsevier B.V. 保留所有权利。

To study the cross-border regulation of immunity and energy metabolism by ginseng miRNA156, and to provide a new perspective for further exploring the possibility of ginseng miRNA156 as a pharmacodynamic substance.Combined with the previous research results of our research group, miRNA156 with high expression in blood sequencing of intragastrically administered with ginseng decoction was selected. Bioinformatics analysis was performed on the selected differential miRNA156. The target genes of differential miRNA156 were mainly enriched in metabolic, immune and other signaling pathways. According to the analysis results, the experimental part will use qi deficiency fatigue model and RAW264.7 cells. The contents of lactic acid (LA), creatine kinase (CK), blood urea nitrogen (BUN), lactate dehydrogenase (LD), liver glycogen (LG), muscle glycogen (MG), interleukin 4 (IL-4), matrix metallo-proteinase 9 (MMP-9), superoxide dismutase (SOD), malondialdehyde, phosphor-enolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G6pase), nitric oxide (NO) and tumor necrosis factor-α (TNF-α) were measured after administration of miRNA156.Ginseng miRNA156 can accelerate the removal of metabolic waste during exercise. Increase the glycogen reserve in, provide energy for the body, regulate the activity of key gluconeogenesis enzyme phosphorus, improve the energy metabolism system of, and enhance the endurance of fatigue mice. The contents of matrix metalloproteinase 9, superoxide dismutase and malondialdehyde were affected, and the content of TNF-α in the supernatant of RAW264.7 cells was significantly increased, which had certain antioxidant capacity and potential immunomodulatory effects.Ginseng miRNA156 has a certain regulatory effect on the energy metabolism and immune function of mice, which makes it possible to regulate the cross-species regulation of ginseng miRNA in theory, provides ideas for ginseng miRNA to become a new pharmacodynamic substance.Copyright © 2024 Elsevier B.V. All rights reserved.