菊粉作为一种天然多糖，可调节肠道微生物，提高免疫和胃肠功能。为了探讨菊粉对结肠癌肺转移的影响，我们建立了CT26注射肺转移模型。结果显示，单独使用菊粉并不能改善结肠癌的肺转移，而与模型组和菊粉组相比，菊粉联合利福昔明显着延长小鼠的生存时间，并抑制肺转移。菊粉处理增加了变形菌门和放线菌门等有害细菌的丰度，而联合处理则降低了它们的丰度，并增加了属于胆汁酸相关细菌的厚壁菌门和真杆菌门等有益菌的丰度。联合治疗降低了小鼠粪便中初级胆汁酸和次级胆汁酸的含量，特别是TGR5激动剂DCA和LCA的含量。此外，联合治疗降低了TGR5、细胞周期蛋白依赖性激酶4、细胞周期蛋白1和CDK2的mRNA表达，增加了肺中p21的mRNA表达，下调了NF-κB p65的水平，并上调了肺中p21的mRNA表达。 TNF-α与模型组比较。以上可能是联合治疗效果更好的原因。版权所有 © 2024. Published by Elsevier B.V.

Inulin as a natural polysaccharide regulates intestinal microorganisms, and improves the immune and gastrointestinal function. In order to explore the effect of inulin on pulmonary metastasis of colon cancer, we set up a CT26 injected pulmonary metastatic model. The results showed that inulin used alone did not improve pulmonary metastasis of colon cancer, while inulin combined with rifaximin significantly prolonged the survival time of mice, and inhibited pulmonary metastasis compared with model and inulin groups. Inulin treatment increased the abundance of harmful bacteria such as Proteobacteria and Actinobacteria, while combined treatment decreased their abundance and increased the abundance of beneficial bacteria containing Firmicutes and Eubacterium which belonged to the bile acid-related bacteria. The combination treatment decreased the content of primary bile acids and secondary bile acids in the feces of mice, especial for DCA and LCA which were the agonists of TGR5. Furthermore, the combination treatment reduced the mRNA expression of the TGR5, cyclin dependent kinase 4, cyclin 1 and CDK2, increased the mRNA expression of p21 in the lung, down-regulated the level of NF-κB p65, and up-regulated the level of TNF-α compared with the model group. The above may be the reason for the better use of the combination treatment.Copyright © 2024. Published by Elsevier B.V.