骨肉瘤（OS）恶性程度高，易发生局部浸润和远处转移。由于OS患者的预后较差，本研究旨在识别OS中的差异表达基因（DEG）并探讨其在OS癌发生和进展中的作用。通过RNA测序来识别OS中的DEG。使用生物信息学分析研究 OS 中 DEG 的功能，并使用 RT-qPCR 和 Western blotting 验证 DEG 表达。使用基因集富集分析 (GSEA) 评估 SLC25A4 的作用，然后使用 OS 细胞中的功能测定进行研究。总共筛选了 8353 个 DEG。 GO和KEGG富集分析表明这些DEG在钙信号通路和癌症通路中表现出强烈的富集。此外，Kaplan-Meier 生存分析显示 10 个中心基因与 OS 患者的预后相关。 OS中SLC25A4转录物和蛋白表达均显着降低，GSEA提示SLC25A4与细胞周期、细胞凋亡和炎症相关。 SLC25A4过表达的OS细胞表现出增殖、迁移、侵袭受到抑制和细胞凋亡增强。发现SLC25A4在OS患者中显着下调，这与不良预后相关。 SLC25A4 表达水平的调节可能有益于 OS 治疗。版权所有 © 2024 张、王、张、冯、兴、王、黄、李和张。

Osteosarcoma (OS) is highly malignant and prone to local infiltration and distant metastasis. Due to the poor outcomes of OS patients, the study aimed to identify differentially expressed genes (DEGs) in OS and explore their role in the carcinogenesis and progression of OS.RNA sequencing was performed to identify DEGs in OS. The functions of the DEGs in OS were investigated using bioinformatics analysis, and DEG expression was verified using RT-qPCR and Western blotting. The role of SLC25A4 was evaluated using gene set enrichment analysis (GSEA) and then investigated using functional assays in OS cells.In all, 8353 DEGs were screened. GO and KEGG enrichment analyses indicated these DEGs showed strong enrichment in the calcium signaling pathway and pathways in cancer. Moreover, the Kaplan-Meier survival analysis showed ten hub genes were related to the outcomes of OS patients. Both SLC25A4 transcript and protein expression were significantly reduced in OS, and GSEA suggested that SLC25A4 was associated with cell cycle, apoptosis and inflammation. SLC25A4-overexpressing OS cells exhibited suppressed proliferation, migration, invasion and enhanced apoptosis.SLC25A4 was found to be significantly downregulated in OS patients, which was associated with poor prognosis. Modulation of SLC25A4 expression levels may be beneficial in OS treatment.Copyright © 2024 Zhang, Wang, Zhang, Feng, Xing, Wang, Huang, Li and Zhang.