这项研究调查了内生真菌生产紫杉醇 (Taxol®) 的潜力，紫杉醇是一种广泛用于化疗的有效抗癌化合物。本研究旨在鉴定、确认和表征能够生产紫杉醇 (PTX) 的内生真菌，并评估其紫杉醇产量。此外，其目的还在于研究影响紫杉醇生产的因素。从紫蒿根中收集并鉴定了总共100株内生真菌分离株。烟曲霉在分离的内生真菌中表现出最高的 PTX 产量（26.373 μg L-1）。该菌株被鉴定为A. fumigatiaffinis（保藏号PP235788.1）。分子鉴定证实了其新颖性，这是首次报道紫蒿内生菌 A. fumigatiaffinis 产生 PTX。通过全因子实验设计 (DOE) 和响应面方法 (RSM) 进行优化，在 pH 8.0、150 μg L-1 苯辅酶的最佳条件下，将 1 g 真菌培养物干重的 PTX 产量显着提高至 110.23 μg L-1补充，并在马铃薯葡萄糖肉汤中发酵 18 天。使用薄层色谱法、高效液相色谱法和气相色谱-质谱法证实紫杉醇的存在。这些发现最大限度地发挥内生真菌产生次级代谢产物的作用，这种次级代谢产物可能能够替代化学方法生产的 PTX，并为提供高浓度、环保的 PTX 可持续来源提供了机会。要点： • 烟曲霉等内生真菌有望用于环保型紫杉醇生产 • 优化策略显着提高紫杉醇产量，达到 110.23 μg L -1 • 分子鉴定证实了新颖性，提供了可持续的 PTX 来源。© 2024。作者。

This study investigated the potential of endophytic fungi to produce paclitaxel (Taxol®), a potent anticancer compound widely employed in chemotherapy. This research aimed to identify, confirm, and characterize endophytic fungi capable of paclitaxel (PTX) production and assess their paclitaxel yield. Additionally, it aimed to investigate factors influencing paclitaxel production. A total of 100 endophytic fungal isolates were collected and identified from the roots of Artemisia judaica. Aspergillus fumigatiaffinis exhibited the highest PTX production (26.373 μg L-1) among the isolated endophytic fungi. The strain was identified as A. fumigatiaffinis (Accession No. PP235788.1). Molecular identification confirmed its novelty, representing the first report of PTX production by A. fumigatiaffinis, an endophyte of Artemisia judaica. Optimization through full factorial design of experiments (DOE) and response surface methodology (RSM) significantly enhanced PTX production to 110.23 μg L-1 from 1 g of dry weight of the fungal culture under optimal conditions of pH 8.0, 150 μg L-1 becozyme supplementation, and 18 days of fermentation in potato dextrose broth. The presence of paclitaxel was confirmed using thin layer chromatography, high performance liquid chromatography, and gas chromatography-mass spectrometry. These findings maximize the role of endophytic fungus to produce a secondary metabolite that might be able to replace the chemically produced PTX and gives an opportunity to provide a sustainable source of PTX eco-friendly at high concentrations. KEY POINTS: • Endophytic fungi, like A. fumigatiaffinis, show promise for eco-friendly paclitaxel production • Optimization strategies boost paclitaxel yield significantly, reaching 110.23 μg L -1 • Molecular identification confirms novelty, offering a sustainable PTX source.© 2024. The Author(s).