研究动态
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霸王中山奈酚衍生物的提取及其多种生物活性。

Extraction of kaempferol derivatives from Zygophyllum paulayanum and its diverse biological activities.

发表日期:2024 Jul 03
作者: Mohan Lal, Dipankar Sutradhar
来源: ANTIOXIDANTS & REDOX SIGNALING

摘要:

霸王(Zygophylllaceae)是一种常见于沙漠地区的植物,以其抗氧化、抗癌、伤口愈合、抗炎和抗菌等特性而闻名。在目前的工作中,我们研究了从泡乌中提取山奈酚衍生物,该衍生物表现出优异的生物活性。使用乙醇提取整个植物(根、叶和茎),用HCl水解,并研究活性分子的鉴定。 TLC、HPLC 和 LCMS 等不同技术已用于鉴定和确认山奈酚苷元类黄酮。基于电喷雾电离的质谱方法已证实山奈酚类黄酮的存在。除了水解提取物外,还对未水解提取物进行了 LCMS 测试,证实存在糖苷,例如山奈酚 3-O-β-D-吡喃葡萄糖基-7-O-α-L-吡喃鼠李糖苷、山奈酚 3-O-β -芸香苷和山奈酚-3-邻鼠李糖苷。泡椒的两种提取物均表现出优异的抗氧化、抗炎、抗菌、植物雌激素和细胞毒性特性,这可能是由于山奈酚衍生物的存在。
Zygophyllum paulayanum (Zygophyllaceae), is a plant commonly found in the desert region, well-known for its antioxidant, anticancer, wound healing, anti-inflammatory and antibacterial, properties. In this present work, we have studied the extraction of kaempferol derivatives from Z. paulayanum which showed excellent biological activities. The whole plant (root, leaves and stem) was extracted using ethanol, hydrolysed with HCl, and studied for the identification of active molecules. Different techniques like TLC, HPLC, and LCMS have been used to identify and confirm the kaempferol aglycone flavonoid. A mass spectrometric method based on electrospray ionisation has confirmed the presence of kaempferol flavonoid. Apart from the hydrolysed extract, the unhydrolyzed extract was also tested for LCMS which confirms the presence of glycosides such as kaempferol 3-O-beta-D-glucopyranosyl-7-O-alpha-L-rhamnopyranoside, kaempferol 3-O-β -rutinoside and kaempferol-3-o-rhamnoside. Both extracts of Z. paulayanum exhibited superior antioxidant, anti-inflammatory, antimicrobial, phytoestrogenic and cytotoxic properties which might be due to the presence of kaempferol derivatives.