这项研究强调了从 MEP（甲基赤藓糖醇 4-磷酸）途径过度表达 1-脱氧-d-木酮糖-5-磷酸合酶 (DXS) 以及短链异戊烯基转移酶融合对于提高二萜类群生产的重要性-4,11-二烯，第一个用于生产抗癌药物紫杉醇的中间体。结果表明，该菌株具有（i）紫杉二烯合酶（txs）基因整合到基因组中，（ii）MEP途径基因过表达，（iii）fpps-crtE异戊烯基转移酶融合蛋白和（iv）额外表达1 -脱氧-d-木酮糖-5-磷酸合酶 (DXS) 产生最高的分类群 4,11-二烯产量，为 390mg/L (26mg/L/OD600)。与枯草芽孢杆菌中报告的最高浓度相比，这意味着增加了十三倍。对 DXS 额外过度表达和利用短链异戊二烯基转移酶融合的关注强调了它们在实现萜烯生物合成效价显着提高方面的关键作用。版权所有 © 2024。由 Elsevier B.V. 出版。

This study highlights the significance of overexpressing 1-deoxy-d-xylulose-5-phosphate synthase (DXS) from the MEP (methylerythritol 4-phosphate) pathway, in addition to short-chain prenyltransferase fusions for the improved production of the diterpene, taxa-4,11-diene, the first committed intermediate in the production of anti-cancer drug paclitaxel. The results showed that the strain which has (i) the taxadiene synthase (txs) gene integrated into the genome, (ii) the MEP pathway genes overexpressed, (iii) the fpps-crtE prenyltransferases fusion protein and (iv) additional expression of 1-deoxy-d-xylulose-5-phosphate synthase (DXS), yielded the highest production of taxa-4,11-diene at 390mg/L (26mg/L/OD600). This represents a thirteen-fold increase compared to the highest reported concentration in B. subtilis. The focus on additional overexpression of DXS and utilizing short-chain prenyltransferase fusions underscores their pivotal role in achieving significant titer improvements in terpene biosynthesis.Copyright © 2024. Published by Elsevier B.V.