在医学诊断中，仅依靠一种生物标志物不足以准确识别癌症。基于血液的多种癌症早期检测可以帮助从单一血液样本中识别出不止一种类型的癌症。在这项研究中，开发了一种基于三种不同颜色的量子点（QD）与链霉亲和素缀合的超分辨率多光谱成像纳米免疫传感器（srMINI），用于在单分子水平上同时筛查血液中的各种癌症生物标志物。实验中，srMINI芯片用于同时检测三种关键的癌症生物标志物：癌胚抗原（CEA）、C反应蛋白（CRP）和甲胎蛋白（AFP）。由于不存在来自底物的干扰信号，srMINI 芯片对这些癌症生物标志物的检测灵敏度比商业酶联免疫吸附测定试剂盒高 108 倍，为 0.18-0.5 ag/mL (1.1-2.6 zM)，这为多重检测奠定了巨大的潜力检测血液中的癌症生物标志物。因此，使用所开发的具有高诊断精度和准确性的srMINI芯片同时检测各种癌症生物标志物，有望作为单分子生物传感器在早期诊断或社区筛查中发挥决定性作用。

In medical diagnosis, relying on only one type of biomarker is insufficient to accurately identify cancer. Blood-based multicancer early detection can help identify more than one type of cancer from a single blood sample. In this study, a super-resolution multispectral imaging nanoimmunosensor (srMINI) based on three quantum dots (QDs) of different color conjugated with streptavidin was developed for the simultaneous screening of various cancer biomarkers in blood at the single-molecule level. In the experiment, the srMINI chip was used to simultaneously detect three key cancer biomarkers: carcinoembryonic antigen (CEA), C-reactive protein (CRP), and alpha-fetoprotein (AFP). The srMINI chip exhibited 108 times higher detection sensitivity of 0.18-0.5 ag/mL (1.1-2.6 zM) for these cancer biomarkers than commercial enzyme-linked immunosorbent assay kits because of the absence of interfering signals from the substrate, establishing considerable potential for multiplex detection of cancer biomarkers in blood. Therefore, the simultaneous detection of various cancer biomarkers using the developed srMINI chip with high diagnostic precision and accuracy is expected to play a decisive role in early diagnosis or community screening as a single-molecule biosensor.