本研究涉及一种称为根皮素的多酚化合物。根皮素 (Ph) 是一种二氢查尔酮，对伊拉克苹果（苹果）“Ibrahimi”的不同地上部分（包括叶、叶柄、茎、果肉和果皮提取物）进行定性和定量测定。叶子是 Ph 的丰富来源，可通过制备型 HPLC 进行分离和纯化。使用各种分析表征技术确认了分离的根皮素 (Ph2) 的化学结构：TLC、HPLC、FTIR、熔点、CHN 元素分析、1H-NMR 和 13C-NMR。采用DPPH测定法测定Ph2的清除效力。通过 MTT 测定评估对癌细胞系的细胞毒性作用，包括（Hep G2/人肝细胞癌、A549/人肺腺癌、SW480/人结肠癌细胞和 AGS/胃腺癌），以及非癌细胞系（赫克293）。从 18.146 克苹果叶中获得约 1.404 克 Ph2（7.7%）。 DPPH和MTT测定结果表明，纯化的Ph2具有有效的抗氧化活性，对所有癌细胞系均具有显着的抗癌作用。数据表明，从伊拉克苹果叶中纯化的Ph2具有潜在的抗氧化、细胞毒性，可能有益于人类健康。

The present study was dealing with a Polyphenolic compound known as Phloretin. Phloretin (Ph), a dihydrochalcone, was determined qualitatively and quantitatively in different aerial parts for Iraqi Malus domestica (apple), cv." Ibrahimi" included leaves, petioles, stems, fruit pulp, and peels extracts. Leaves represented a rich source of Ph, which was separated and purified by preparative HPLC. The chemical structure of the isolated Phloretin (Ph2) was confirmed using various analytical characterization techniques: TLC, HPLC, FTIR, Melting point, CHN elemental analyses, 1H-NMR, and 13C-NMR). The scavenging efficacy of Ph2 by DPPH assay was employed. Cytotoxic effect was assessed by MTT assay against cancer cell lines including (Hep G2/ human hepatocyte carcinoma, A549/ human lung adenocarcinoma, SW480 / human colon cancer cell, and AGS /adenocarcinoma of the stomach), beside the non-cancerous cell line (HEK 293). About 1.404 g Ph2 was obtained from 18.146 g apple leaves (7.7%). The DPPH and MTT assay results demonstrated that the purified Ph2 possessed potent antioxidant activity with significant anticancer effects on all cancer cell lines. Data suggested that purified Ph2 from Iraqi apple leaves has potential antioxidant, cytotoxicity, which may benefit in human health.