陈皮（CRP）是常用的保健食品和中药，具有抗心血管、抗肿瘤、抗氧化、抗炎、抗病毒、保肝等药理作用。 、降血压和神经保护作用。在本研究中，开发并验证了可靠、灵敏的超高效液相色谱/串联质谱 (UPLC-MS/MS) 和气相色谱-质谱 (GC-MS) 方法，用于测定大鼠体内的 11 种活性成分口服CRP提取物后的血浆。该方法的结果表明，特异性、线性（r > 0.999）、精密度和准确度（变异系数（CV）< 11.5%）、回收率（52.9-107.9%）、基质效应（63.8-107.5%）、稳定性（CV < 10.8%）满足血浆样品定量的所有要求。药动学结果显示，黄酮苷的达峰时间小于0.7小时，多甲氧基黄酮及挥发性成分的达峰时间在1-7小时内。同时，橙皮苷、川陈皮素、橘皮素和D-柠檬烯的曲线下面积（AUC）和最大浓度（Cmax）均高于其他成分，表明这些成分的血浆暴露水平较高在 CRP 中。本研究为阐明CRP的治疗物质基础奠定了基础，为进一步的科学研究和临床应用提供参考。Copyright © 2024 Elsevier B.V. 版权所有。

Citri Reticulatae Pericarpium (CRP) is used as common health-care food and traditional Chinese medicine (TCM), which exerts pharmacological effects, such as anti-cardiovascular, anti-tumor, anti-oxidant, anti-inflammatory, anti-virus, hepatoprotective, blood pressure-lowering and neuroprotective. In this study, reliable, and sensitive ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) and gas chromatography-mass spectrometry (GC-MS) methods were developed and validated for the determination of eleven active components in rat plasma after oral administration of the CRP extract. The results of this method exhibited that the specificity, linearity (r > 0.999), precision and accuracy (the coefficient of variation (CV) < 11.5 %), recovery (52.9-107.9 %), matrix effects (63.8-107.5 %), and stability (CV < 10.8 %) met all requirements for the quantitation of plasma samples. The pharmacokinetic results showed that the Tmax of flavone glycosides was less than 0.7 h, and that of polymethoxyflavones and volatile components were within 1-7 h. Meanwhile, the area-under-the-curve (AUC) and concentration maximum (Cmax) of hesperidin, nobiletin, tangeretin, and D-limonene were higher than those of the other components, suggesting that the plasma exposure levels of these constituents were higher in CRP. The present research lays a foundation for elucidating the therapeutic material basis and provides a reference for further scientific research and clinical application of CRP.Copyright © 2024 Elsevier B.V. All rights reserved.