信使 RNA (mRNA) 已成为一种有前途的治疗分子，在治疗中枢神经系统疾病、肿瘤、COVID-19 和其他疾病方面具有广泛的临床应用。 mRNA 疗法必须封装到安全、稳定和有效的递送载体中，以防止货物降解并防止免疫原性。外泌体因其良好的生物相容性、低免疫原性、小尺寸、独特的穿越生理屏障的能力和细胞特异性向性而在 mRNA 递送中受到越来越多的关注。此外，这些外泌体可以被设计为利用天然载体来靶向特定的细胞或组织。这种有针对性的方法将增强 mRNA 的功效并减少副作用。然而，必须解决诸如缺乏一致可靠的外泌体纯化方法以及将大mRNA有效封装到外泌体中等困难。本文概述了细胞源性囊泡介导的 mRNA 递送及其生物医学应用的当前突破。© 2024。作者。

Messenger RNA (mRNA) has emerged as a promising therapeutic molecule with numerous clinical applications in treating central nervous system disorders, tumors, COVID-19, and other diseases. mRNA therapies must be encapsulated into safe, stable, and effective delivery vehicles to preserve the cargo from degradation and prevent immunogenicity. Exosomes have gained growing attention in mRNA delivery because of their good biocompatibility, low immunogenicity, small size, unique capacity to traverse physiological barriers, and cell-specific tropism. Moreover, these exosomes can be engineered to utilize the natural carriers to target specific cells or tissues. This targeted approach will enhance the efficacy and reduce the side effects of mRNAs. However, difficulties such as a lack of consistent and reliable methods for exosome purification and the efficient encapsulation of large mRNAs into exosomes must be addressed. This article outlines current breakthroughs in cell-derived vesicle-mediated mRNA delivery and its biomedical applications.© 2024. The Author(s).