恶病质是一种在非小细胞肺癌患者中发病率较高的综合征，它会损害生活质量，降低对癌症治疗的耐受性和反应性，导致生存率下降。最佳营养护理对于恶病质的治疗至关重要，也是多模式治疗的推荐基石。在这里，我们研究了由高蛋白、亮氨酸、鱼油、维生素 D、低聚半乳糖和低聚果糖的特定组合组成的干预饮食对原位肺癌小鼠模型恶病质发生和进展的治疗效果11周龄雄性129S2/Sv小鼠原位植入344P肺上皮肿瘤细胞或载体（对照）。植入后 7 天，荷瘤 (TB) 小鼠被分配至干预饮食或等热量对照饮食。恶病质被定义为连续 5 天体重减轻，之后对小鼠进行安乐死进行组织分析。与假手术小鼠相比，结核病小鼠出现恶病质，并伴有骨骼肌质量和附睾脂肪量显着减少。恶病质终点显着延迟（46.0 ± 15.2 对比 34.7 ± 11.4 天），数量（-1.57 ± 0.62 对比 -2.13 ± 0.57 克）和进展（-0.26 ± 0.14 对比 -0.39 ± 0.11 克/天）与对照饮食相比，干预措施显着减少了体重减轻。此外，干预饮食在骨骼肌中抑制了全身炎症（pentraxin-2血浆水平）以及蛋白水解和蛋白质合成分子标记物的改变（表明结核病小鼠的肌肉萎缩信号传导）。 总之，这些数据证明了这种多营养素干预措施针对恶病质的多种成分，作为肺癌管理的组成部分。© 2024 作者。 《恶病质、肌肉减少症和肌肉杂志》由 Wiley periodicals LLC 出版。

Cachexia, a syndrome with high prevalence in non-small cell lung cancer patients, impairs quality of life and reduces tolerance and responsiveness to cancer therapy resulting in decreased survival. Optimal nutritional care is pivotal in the treatment of cachexia and a recommended cornerstone of multimodal therapy. Here, we investigated the therapeutic effect of an intervention diet consisting of a specific combination of high protein, leucine, fish oil, vitamin D, galacto-oligosaccharides, and fructo-oligosaccharides on the development and progression of cachexia in an orthotopic lung cancer mouse model.Eleven-week-old male 129S2/Sv mice were orthotopically implanted with 344P lung epithelial tumour cells or vehicle (control). Seven days post-implantation tumour-bearing (TB) mice were allocated to either intervention- or isocaloric control diet. Cachexia was defined as 5 days of consecutive body weight loss, after which mice were euthanized for tissue analyses.TB mice developed cachexia accompanied by significant loss of skeletal muscle mass and epididymal fat mass compared with sham operated mice. The cachectic endpoint was significantly delayed (46.0 ± 15.2 vs. 34.7 ± 11.4 days), and the amount (-1.57 ± 0.62 vs. -2.13 ± 0.57 g) and progression (-0.26 ± 0.14 vs. -0.39 ± 0.11 g/day) of body weight loss were significantly reduced by the intervention compared with control diet. Moreover, systemic inflammation (pentraxin-2 plasma levels) and alterations in molecular markers for proteolysis and protein synthesis, indicative of muscle atrophy signalling in TB-mice, were suppressed in skeletal muscle by the intervention diet.Together, these data demonstrate the potential of this multinutrient intervention, targeting multiple components of cachexia, as integral part of lung cancer management.© 2024 The Author(s). Journal of Cachexia, Sarcopenia and Muscle published by Wiley Periodicals LLC.