水飞蓟宾具有抗肿瘤活性。然而，关于水飞蓟宾对肿瘤微环境中T淋巴细胞功能的免疫调节作用的报道很少。在此，我们确定了水飞蓟宾对外周血单核细胞 (PBMC) 的 T 细胞的影响，单独培养或与人胶质母细胞瘤细胞系 (U-87 MG) 一起培养。通过 MTT 测试评估 T 淋巴细胞的增殖情况。水飞蓟宾（15 和 45 µM）。此外，还比较了单独治疗和未治疗的 PBMC 的总抗氧化能力 (TAC)、超氧化物歧化酶 3 (SOD3) 活性以及两种细胞因子干扰素 γ (IFN-γ) 和肿瘤生长 β (TGF-β) 的水平或与U-87细胞共培养。根据我们的结果，水飞蓟宾提高了PBMC和共培养条件下的TAC水平和SOD3活性。此外，水飞蓟宾处理的PBMC表现出较高的IFN-γ水平和较低的TGF-β水平。有趣的是，水飞蓟宾保护PBMC免受U-87诱导的抑制。总之，这些结果提出了水飞蓟宾对PBMC T细胞的免疫调节潜力，以及它对PBMC免受U-87 MG细胞诱导的抑制的部分保护作用。

Silibinin has exhibited antitumor activities. However, there are few reports about the immunomodulatory properties of silibinin on T lymphocyte function in the tumor microenvironment. Here, we determined the effects of silibinin on T cells of peripheral blood mononuclear cells (PBMCs), cultivated alone or with a human cell line of glioblastoma (U-87 MG).The proliferation of T lymphocytes was assessed by MTT test in the presence of silibinin (15 and 45 µM). Also, total antioxidant capacity (TAC), the activity of superoxide dismutase-3 (SOD3), and the levels of two cytokines interferon gamma (IFN-γ) and tumor growth beta (TGF-β) were compared between treated and untreated PBMCs alone or co-cultured with U-87 cells.According to our results, silibinin raised the TAC levels and SOD3 activity in the PBMCs and in the co-culture condition. Moreover, silibinin-treated PBMCs showed higher IFN-γ levels and lower TGF-β levels. Interestingly, silibinin protected PBMCs against the U-87-induced suppression.Altogether, these results proposed the immunomodulatory potential of silibinin on T cells of PBMCs, as well as its partially protective effects on PBMCs against the suppression induced by U-87 MG cells.