葡萄膜黑色素瘤（UM）是一种高度侵袭性和致命性的眼部肿瘤，由于UM的特殊生物学特性，免疫治疗对UM患者效果甚微。提高UM患者免疫治疗的疗效具有重要的临床意义。单细胞 RNA 测序 (scRNA-seq) 为破译肿瘤内异质性和肿瘤微环境 (TME) 的复杂性提供了关键视角。结合生物信息学分析，scRNA-seq可以帮助寻找与预后相关的分子指标，开发新的治疗靶点，特别是免疫治疗，最终指导临床治疗选择。版权所有©2024唐，张，黄，朱和黄。

Uveal melanoma (UM) is a highly aggressive and fatal tumor in the eye, and due the special biology of UM, immunotherapy showed little effect in UM patients. To improve the efficacy of immunotherapy for UM patients is of great clinical importance. Single-cell RNA sequencing(scRNA-seq) provides a critical perspective for deciphering the complexity of intratumor heterogeneity and tumor microenvironment(TME). Combing the bioinformatics analysis, scRNA-seq could help to find prognosis-related molecular indicators, develop new therapeutic targets especially for immunotherapy, and finally to guide the clinical treatment options.Copyright © 2024 Tang, Zhang, Huang, Zhu and Huang.