灵芝或灵芝，Ganoderma lucidum，是一种作为草药而得到广泛开发的药用蘑菇，因为它具有抗癌、抗肿瘤、抗氧化和抗炎作用。灵芝中的活性真菌化学成分，如黄酮类和多糖，可以抑制促炎细胞因子的释放，并防止氧化应激引起的脂质过氧化。类风湿性关节炎（RA）是一种自身免疫性疾病，其确切原因尚不清楚，并且 RA 患病率逐年增加。 RA 患者会出现关节损伤和炎症。本研究旨在评估灵芝纳米凝胶抗关节炎、抗炎和抗氧化的有效性。研究方法为真实实验，采用随机分配的对照组和治疗组，使用24只雄性Wistar大鼠（Rattusnorvegicus），用完全弗氏佐剂（CFA）0.1mL诱导。将大鼠分为六组；健康对照/HCt（未接受治疗）、阴性对照/NCt（由CFA诱导）和阳性对照/PCt（给予0.012双氯芬酸钠）。 TG1（给予250mg灵芝纳米凝胶）、TG2（给予500mg灵芝纳米凝胶）、TG3（给予750mg灵芝纳米凝胶）。使用ELISA测量IgG、eNOS、IL-1β、COX-2、NOS、TNF-α和IL-6参数，并使用SPSS对获得的数据进行单向方差分析（P < 0.05）。结果显示，服用灵芝纳米凝胶可显着降低 IgG、NOS、TNF-α、COX-2、IL-1β 和 IL-6，并增加 eNOS 水平。抑制促炎细胞因子和增加 eNOS 水平的抗炎和抗氧化活性证明纳米凝胶提取物灵芝具有开发为抗关节炎天然疗法的潜力。

Lingzhi or reishi mushroom, Ganoderma lucidum, is a medicinal mushroom quite widely developed as herbal medicine because it has acted as an anticancer, antitumor, antioxidant, and anti-inflammatory. The active mycochemical compounds of G. lucidum mushrooms, such as flavonoids and polysaccharides, can suppress the release of pro-inflammatory cytokines and prevent lipid peroxidation due to oxidative stress. Rheumatoid arthritis (RA) is an autoimmune disease where the exact cause is unknown, and RA prevalence continues to increase yearly. In patients with RA, joint damage and inflammation occur. This study aims to evaluate the effectiveness of G. lucidum nanogels as anti-arthritis, anti-inflammatory, and antioxidative. The research method was a true experiment using a control group and treatment group that randomly assigned, using 24 male Wistar rats (Rattus norvegicus) induced with complete Freund's adjuvant (CFA) 0.1 mL. The rats were divided into six groups; healthy control/HCt (did not receive the treatment), negative control/NCt (induced by CFA), and positive control/PCt (given 0.012 diclofenac sodium). TG1 (given 250 mg G. lucidum nanogels), TG2 (given 500 mg G. lucidum nanogels), TG3 (given 750 mg G. lucidum nanogels). IgG, eNOS, IL-1β, COX-2, NOS, TNF-α, and IL-6 parameters were measured using ELISA, and the data obtained were analyzed by one-way ANOVA using SPSS (P < 0.05). The results showed that administering G. lucidum nanogels significantly reduced IgG, NOS, TNF-α, COX-2, IL-1β, and IL-6 and increased eNOS levels. The anti-inflammatory and antioxidative activities in suppressing pro-inflammatory cytokines and increasing eNOS levels prove that the nanogel extract G. lucidum have the potential to be developed as anti-arthritis natural therapeutic.