谷物的嫩芽被广泛认为是具有健康益处的超级食品，因为它们具有潜在的抗氧化活性和抗氧化相关作用（例如抗癌）。当前的研究旨在检查大麦甲醇提取物和水提取物的化学特性，并使用 DDPH 和 ORAC 评估其抗氧化活性。此外，还筛选了黄嘌呤氧化酶的抑制作用。采用薄层色谱生物自显影法来确定提取物中表现出最有效的自由基清除活性的化合物的极性。甲醇提取物和水提取物的总黄酮含量分别为 0.14 mg QE/g 和 0.012 mg QE/g。发现甲醇提取物的抗氧化活性比没有活性的水提取物更有效，其值为 0.97±0.13 mmol TE/g。这项研究提出了关于 H. vulgare 黄嘌呤抑制活性的新发现。甲醇提取物对黄嘌呤氧化酶有中等程度的抑制作用，抑制率为23.24%。我们的研究结果与小麦的植物化学和药理分析进行了比较，并与文献报道的数据进行了进一步的比较。观察到 H. vulgare 的化学和药理特性不一致，这可能是由于使用在不同生长期收获的草药材料和不同的提取方法造成的。我们的研究结果表明，收获时机和提取方法可能在获得 H. vulgare 的最佳植物化学成分方面发挥着至关重要的作用，从而增强其药理活性。© 2024。作者。

Young shoots of cereals are widely regarded as superfoods with health benefits attributed to their potential antioxidant activity and antioxidant-related effects (e.g. anticancer). The current study aimed to examine the chemical characteristics of Hordeum vulgare methanolic and aqueous extracts and assess their antioxidant activity using the DDPH and ORAC. Furthermore, the inhibitory effect of xanthine oxidase was screened. TLC bioautography was employed to determine the polarity of the compounds present in the extracts that exhibited the most potent free radical scavenging activity. Total flavonoid content of the methanolic and aqueous extracts was 0.14 mg QE/g and 0.012 mg QE/g, respectively. The antioxidant activity of the methanolic extract was found to be more potent, with a value of 0.97 ± 0.13 mmol TE/g than the aqueous extract which had no activity. This study presents novel findings on the xanthine inhibitory activity of H. vulgare. The methanolic extract demonstrated moderate inhibition of xanthine oxidase with a value of 23.24%. The results of our study were compared with the phytochemical and pharmacological analysis of Triticum aestivum, and further comparison was made with the data reported in the literature. Inconsistencies were observed in the chemical and pharmacological properties of H. vulgare, which could be a result of using herbal material harvested in different vegetative phases and various methods used for extraction. The findings of our study indicate that the timing of the harvest and extraction method may play crucial role in attaining the optimal phytochemical composition of H. vulgare, hence enhancing its pharmacological activity.© 2024. The Author(s).