据报道，在全球所有类型的头颈癌中，喉癌（LC）的发病率较高。由于涉及侵袭和转移，LC的发病机制非常复杂，需要了解这个复杂的多步骤过程。包括基质金属蛋白酶 (MMP) 在内的许多分子都参与调节转移机制。此外，在炎症、侵袭和转移等多种病理和生理事件中观察到不同类别的 MMP 的激活和表达。在 MMP 的所有成员中，基质金属蛋白酶 2 (MMP-2) 和基质金属蛋白酶 9 (MMP-9) 经常被报道与肿瘤发病机制相关。本研究旨在检查 MMP-2 和 MMP-9 在 LC 发病机制中的参与情况。收集184个喉肿瘤样本以及邻近的未受累健康切片，使用实时PCR和免疫组织化学（IHC）检查LC中上述基因的表达失调。实时 PCR 和 IHC 分析显示，与对照相比，喉肿瘤中 MMP-2 (P < .0001) 和 MMP-9 (P < .0001) 基因显着上调。 Spearman 相关性显示所选 MMP 的表达失调与晚期 TNM 分期呈正相关 [MMP-2，(P < .0001)； MMP-9，P < .0001] 和吸烟状况 [MMP-2 (P < .0001)； MMP-9 P < .0001]在喉部发病机制中的作用。受试者工作曲线（ROC）分析显示所述标志物对喉癌患者具有良好的诊断/预后价值。本研究表明，选定的 MMP 的显着上调与喉癌风险增加相关，并且可以作为检测所述疾病的良好诊断标记。版权所有 © 2024 作者。由 Wolters Kluwer Health, Inc. 出版

Laryngeal carcinoma (LC) is reported to have a higher incidence rate among all types of head and neck cancers around the globe. Mechanisms resulting in the pathogenesis of LC are complicated due to involvement of invasion and metastasis and there is a need to understand this complicated multistep process. Numerous molecules including matrix metalloproteinases (MMPs) are involved in regulating metastatic mechanisms. Furthermore, activation and expression of different classes of MMPs have been observed in multiple pathological and physiological events including inflammation, invasion, and metastasis. Among all members of MMPs, matrix metalloproteinases-2 (MMP-2), and matrix metalloproteinases-9 (MMP-9) have been frequently reported to correlate with tumor pathogenesis. The present study is designed to check the involvement of MMP-2 and MMP-9 in LC pathogenesis. 184 laryngeal tumor samples along with adjacent uninvolved healthy sections were collected to check the expression deregulation of the above-mentioned gene in LC using real-time PCR and immunohistochemistry (IHC). Real-time PCR and IHC analyses showed the significant upregulation of MMP-2 (P < .0001) and MMP-9 (P < .0001) genes in laryngeal tumors compared to controls. Spearman correlation showed the positive correlation of expression deregulation of selected MMPs with advanced TNM stage [MMP-2, (P < .0001); MMP-9, P < .0001] and smoking status [MMP-2 (P < .0001); MMP-9 P < .0001] in laryngeal pathogenesis. Receiver operating curve (ROC) analysis showed the good diagnostic/prognostic value of said markers in laryngeal cancer patients. The present study showed that significant upregulation of selected MMPs was found associated with an increased risk of laryngeal cancer and can act as good diagnostic markers for the detection of said disease.Copyright © 2024 the Author(s). Published by Wolters Kluwer Health, Inc.