定量 microRNA (miRNA) 检测对于早期乳腺癌诊断和预后至关重要。然而，用于 miRNA 识别的快速稳定的荧光传感仍然具有挑战性。这项工作开发了一种基于 AuNPs 蚀刻的新型无标记检测方法，用于定量检测 miRNA-155。使用种子介导的生长方法，在负载有罗丹明 6G (R6G) 的介孔二氧化硅纳米颗粒 (MSN) 的表面上生长一层 AuNP，然后进行探针附着。在 miRNA-155 存在的情况下，MSN@R6G@AuNP 表面失去了所连接探针的保护，使得 AuNP 容易被盐酸蚀刻。这导致在自由空间中释放显着的荧光信号。 AuNPs的封装有效减少了信号泄漏，而快速蚀刻工艺缩短了检测时间。该策略可实现灵敏、快速的检测，检测范围为 100 fM 至 100 nM，检测限为 2.18 fM，检测时间为 30 分钟。正常人血清中的回收率范围为 99.02% 至 106.34%。这项工作提出了一种简单的生物传感策略，在肿瘤诊断中具有巨大的应用潜力。版权所有 © 2024 Elsevier B.V. 保留所有权利。

Quantitative microRNA (miRNA) detection is crucial for early breast cancer diagnosis and prognosis. However, quick and stable fluorescence sensing for miRNA identification is still challenging. This work developed a novel label-free detection method based on AuNPs etching for quantitatively detecting miRNA-155. A layer of AuNPs was grown on the surface of mesoporous silica nanoparticles (MSN) loaded with Rhodamine 6G (R6G) using seed-mediated growth, followed by probe attachment. In the presence of miRNA-155, the MSN@R6G@AuNP surface loses the protection of the attached probe, rendering AuNPs susceptible to etching by hydrochloric acid. This results in a significant fluorescent signal being released in the free space. The encapsulation with AuNPs effectively reduces signal leakage, while the rapid etching process shortens detection time. This strategy enables sensitive and fast detection with a detection range of 100 fM to 100 nM, a detection limit of 2.18 fM, and a detection time of 30 min. The recovery rate in normal human serum ranges from 99.02 % to 106.34 %. This work presents a simple biosensing strategy with significant potential for application in tumor diagnosis.Copyright © 2024 Elsevier B.V. All rights reserved.