肝细胞癌（HCC）是最常见的恶性肿瘤之一，而一种未表征的蛋白RNF214在HCC中的表达和功能仍不清楚。最近观察到相分离参与了 HCC 的进展。在本研究中，我们研究了 RNF214 在 HCC 中的表达、功能和相分离。我们发现 RNF214 在 HCC 中高表达并与不良预后相关。 RNF214作为癌基因促进HCC的增殖、迁移和转移。从机械角度来看，RNF214 经历了相分离，RNF214 的卷曲螺旋 (CC) 结构域介导了其相分离。此外，CC 结构域对于 RNF214 在 HCC 中的致癌功能是必需的。综上所述，我们的数据表明 RNF214 的相分离促进了 HCC 的进展。 RNF214 可能是 HCC 的潜在生物标志物和治疗靶点。© 2024。作者。

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors, and the expression and function of an uncharacterized protein RNF214 in HCC are still unknown. Phase separation has recently been observed to participate in the progression of HCC. In this study, we investigated the expression, function, and phase separation of RNF214 in HCC. We found that RNF214 was highly expressed in HCC and associated with poor prognosis. RNF214 functioned as an oncogene to promote the proliferation, migration, and metastasis of HCC. Mechanically, RNF214 underwent phase separation, and the coiled-coil (CC) domain of RNF214 mediated its phase separation. Furthermore, the CC domain was necessary for the oncogenic function of RNF214 in HCC. Taken together, our data favored that phase separation of RNF214 promoted the progression of HCC. RNF214 may be a potential biomarker and therapeutic target for HCC.© 2024. The Author(s).