PD-1/PD-L1抑制剂已成为一种有前途的治疗方法。然而，宫颈癌（CC）患者的缓解率低于30%，这与肿瘤微环境（TME）中的免疫抑制成分有关。肿瘤相关巨噬细胞（TAM）作为最重要的免疫细胞之一，参与肿瘤抑制微环境的形成。因此，探讨TAMs对PD-L1表达的调节机制，将为提高疗效提供理论依据。收集CC患者的临床资料和病理组织，采用免疫荧光法检测PD-L1、CD68、CD163的表达情况。免疫组织化学。利用生物信息学分析参与PD-L1调节的巨噬细胞亚型。建立共培养模型，观察TAMs对CC细胞形态、迁移和侵袭功能的影响，以及TAMs对PD-L1的调节机制。肿瘤细胞上PD-L1的表达可以预测患者的不良预后。 PD-L1 表达与 CD163 TAM 浸润之间存在很强的相关性。同样，在生物信息学分析中，PD-L1 表达与 M1/M2 型 TAM 浸润相关。细胞共培养结果显示，M1/M2型TAMs可上调PD-L1表达，尤其是M2型TAMs可通过PI3K/AKT途径上调PD-L1表达。同时，M1/M2型TAMs可影响CC细胞的形态变化，增强CC细胞的迁移和侵袭能力。肿瘤细胞中PD-L1的表达可作为预后因素，且与CD163 TAMs浸润密切相关。此外，M2型TAM可以通过PI3K/AKT通路上调CC细胞中PD-L1的表达，增强迁移和侵袭能力，影响肿瘤进展。© 2024。作者。

PD-1/PD-L1 inhibitors have become a promising therapy. However, the response rate is lower than 30% in patients with cervical cancer (CC), which is related to immunosuppressive components in tumor microenvironment (TME). Tumor-associated macrophages (TAMs), as one of the most important immune cells, are involved in the formation of tumor suppressive microenvironment. Therefore, it will provide a theoretical basis for curative effect improvement about the regulatory mechanism of TAMs on PD-L1 expression.The clinical data and pathological tissues of CC patients were collected, and the expressions of PD-L1, CD68 and CD163 were detected by immunohistochemistry. Bioinformatics was used to analyze the macrophage subtypes involved in PD-L1 regulation. A co-culture model was established to observe the effects of TAMs on the morphology, migration and invasion function of CC cells, and the regulatory mechanism of TAMs on PD-L1.PD-L1 expression on tumor cells could predict the poor prognosis of patients. And there was a strong correlation between PD-L1 expression with CD163+TAMs infiltration. Similarly, PD-L1 expression was associated with M1/M2-type TAMs infiltration in bioinformatics analysis. The results of cell co-culture showed that M1/M2-type TAMs could upregulate PD-L1 expression, especially M2-type TAMs may elevate the PD-L1 expression via PI3K/AKT pathway. Meanwhile, M1/M2-type TAMs can affect the morphological changes, and enhance migration and invasion abilities of CC cells.PD-L1 expression in tumor cells can be used as a prognostic factor and is closely related to CD163+TAMs infiltration. In addition, M2-type TAMs can upregulate PD-L1 expression in CC cells through PI3K/AKT pathway, enhance the migration and invasion capabilities, and affect the tumor progression.© 2024. The Author(s).