高危型人乳头瘤病毒（HR-HPV）感染是宫颈癌发生的重要因素。 HPV18是继HPV16之后第二常见的HR-HPV。本研究利用MEGA11软件分析HPV18 E6-E7和L1基因的变异和系统发育树。使用pamlX 估计E6、E7 和L1 基因的选择压力。此外，分别通过ABCpred服务器和IEDB网站预测了HPV18中L1氨基酸序列的B细胞表位和E6-E7氨基酸序列的T细胞表位。在E6-E7序列中总共发现了9个单核苷酸变异，其中2个是非同义变体，7个是同义变体。 L1序列中鉴定出20个单核苷酸变异，其中11个非同义变异和9个同义变异。系统发育分析表明E6-E7和L1序列均分布在A谱系中。在HPV18 E6、E7和L1序列中，未发现正选择位点。 L1 中的非保守取代 R545C 影响假设的 B 细胞表位。 E6中的S82A和E7中的R53Q两个非保守替换影响了多个假设的T细胞表位。HPV18的序列变异数据可能为华中地区的病毒诊断、宫颈癌的进一步研究和疫苗设计奠定基础。© 2024。作者。

High-risk human papillomavirus (HR-HPV) infection is an important factor for the development of cervical cancer. HPV18 is the second most common HR-HPV after HPV16.In this study, MEGA11 software was used to analyze the variation and phylogenetic tree of HPV18 E6-E7 and L1 genes. The selective pressure to E6, E7 and L1 genes was estimated using pamlX. In addition, the B cell epitopes of L1 amino acid sequences and T cell epitopes of E6-E7 amino acid sequences in HPV18 were predicted by ABCpred server and IEDB website, respectively.A total of 9 single nucleotide variants were found in E6-E7 sequences, of which 2 were nonsynonymous variants and 7 were synonymous variants. Twenty single nucleotide variants were identified in L1 sequence, including 11 nonsynonymous variants and 9 synonymous variants. Phylogenetic analysis showed that E6-E7 and L1 sequences were all distributed in A lineage. In HPV18 E6, E7 and L1 sequences, no positively selected site was found. The nonconservative substitution R545C in L1 affected hypothetical B cell epitope. Two nonconservative substitutions, S82A in E6, and R53Q in E7, impacted multiple hypothetical T cell epitopes.The sequence variation data of HPV18 may lay a foundation for the virus diagnosis, further study of cervical cancer and vaccine design in central China.© 2024. The Author(s).