作为癌症的生物标志物，microRNA的准确、灵敏检测具有重要意义。因此，我们提出了一种表面增强拉曼散射（SERS）/电化学（EC）双模式纳米传感器来灵敏地检测miRNA-141。该纳米传感器采用Au@Ag纳米线作为新型SERS/EC传感平台，具有生物相容性好、响应速度快、灵敏度高等优点。双模式纳米传感器不仅可以有效克服单一信号可靠性不足的问题，而且可以实现检测信号的放大和稳定输出，保证miRNA检测的可靠性和重复性。通过这种传感策略，可以在较宽的线性范围（100 fM 至 50 nM）内检测目标 miRNA-141（SERS 的 LOD 为 18.4 fM，电化学方法的 LOD 为 16.0 fM）。此外，该过程显示出良好的选择性，可以将 miRNA-141 与其他干扰 miRNA 区分开来。对人血清样本的实际分析也证明我们的策略具有良好的可靠性、重复性，在分析检测领域具有广阔的应用前景。© 2024. The Author(s), under Exclusive License to Springer-Verlag GmbH, DE施普林格自然的一部分。

As biomarkers of cancer, the accurate and sensitive detection of microRNAs is of great significance. Therefore, we proposed a surface-enhanced Raman scattering (SERS)/electrochemical (EC) dual-mode nanosensor for sensitively detecting miRNA-141. The nanosensor uses Au@Ag nanowires as a novel SERS/EC sensing platform, which has the advantages of good biocompatibility, fast response, and high sensitivity. The dual-mode nanosensor can not only effectively overcome the problem of insufficient reliability of single signal, but also realize the amplification and stable output of the detection signal, to ensure the reliability and repeatability of miRNA detection. With this sensing strategy, the target miRNA-141 can be detected over a wide linear range (100 fM to 50 nM) (LOD of 18.4 fM for SERS and 16.0 fM for electrochemical methods). In addition, the process shows good selectivity and can distinguish miRNA-141 from other interfering miRNAs. The actual analysis of human serum samples also proves that our strategy has good reliability, repeatability, and has broad application prospects in the field of analysis and detection.© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.