尽管光生物调节疗法（PBMt）可用于减轻恶性疾病的术后副作用，但由于癌症复发和继发性恶性肿瘤的发生的可能性，其应用仍然存在争议。我们研究了PBMt对HT29结肠癌细胞线粒体功能的影响。PBMt后通过MTT测定测定HT29细胞增殖。进行免疫荧光染色以确定线粒体生物发生和活性氧（ROS）。用 Mitotracker 测量线粒体膜电位。进行蛋白质印迹以确定裂变、融合、UCP2以及细胞周期蛋白B1和D1蛋白的表达。体内研究通过将癌细胞皮下接种到裸鼠体内，并进行免疫组织化学测定 FIS1、MFN2、UCP2 和 p-AKT 的表达。使用 PBMt（670 nm，发光二极管）时，HT29 细胞的增殖和迁移达到最大, LED) 与对照 (P < 0.05) 相比，强度为 2.0 J/cm2，细胞周期蛋白 B1 和细胞周期蛋白 D1 的表达更多 (P < 0.05)。免疫荧光染色显示，与对照组相比，PBMt 后 ROS 和线粒体膜电位增强。 PBMt 组线粒体的 ATP 合成也高于对照组（P < 0.05）。 PBMt 组裂变蛋白和融合蛋白的表达水平显着高于对照组（P < 0.05）。电子显微镜显示，两组之间显示裂变的线粒体百分比没有显着差异。 PBMt 组细胞培养上清液中包括 D-2-羟基谷氨酸在内的肿瘤代谢物高于对照组，且 UCP2 表达增加（P < 0.05）。 PBMt组裸鼠模型中的肿瘤大小和异种移植物重量均大于对照组（P < 0.05）。免疫组织学观察，裸鼠异种移植物中线粒体生物发生蛋白 UCP2 和 p-AKT 在 PBMt 组中的表达量高于对照组（P < 0.05）。使用红光 LED 的 PBM 治疗可能通过增加线粒体活动和裂变。版权所有 © 2024 Elsevier B.V. 保留所有权利。

Although photobiomodulation therapy (PBMt) is available to alleviate post-operative side effects of malignant diseases, its application is still controversial due to some potential of cancer recurrence and occurrence of a secondary malignancy. We investigated effect of PBMt on mitochondrial function in HT29 colon cancer cells.HT29 cell proliferation was determined with MTT assay after PBMt. Immunofluorescent staining was performed to determine mitochondrial biogenesis and reactive oxygen species (ROS). Mitochondrial membrane potential was measured with Mitotracker. Western blotting was executed to determine expression of fission, fusion, UCP2, and cyclin B1 and D1 proteins. In vivo study was performed by subcutaneously inoculating cancer cells into nude mice and immunohistochemistry was done to determine expression of FIS1, MFN2, UCP2, and p-AKT.The proliferation and migration of HT29 cells reached maximum with PBMt (670 nm, light emitting diode, LED) at 2.0 J/cm2 compared to control (P < 0.05) with more expression of cyclin B1 and cyclin D1 (P < 0.05). Immunofluorescent staining showed that ROS and mitochondrial membrane potential were enhanced after PBMt compared to control. ATP synthesis of mitochondria was also higher in the PBMt group than in the control (P < 0.05). Expression levels of fission and fusion proteins were significantly increased in the PBMt group than in the control (P < 0.05). Electron microscopy revealed that the percentage of mitochondria showing fission was not significantly different between the two groups. Oncometabolites including D-2-hydoxyglutamate in the supernatant of cell culture were higher in the PBMt group than in the control with increased UCP2 expression (P < 0.05). Both tumor size and weight of xenograft in nude mice model were bigger and heavier in the PBMt group than in the control (P < 0.05). Immunohistologically, mitochondrial biogenesis proteins UCP2 and p-AKT in xenograft of nude mice were expressed more in the PBMt group than in the control (P < 0.05).Treatment with PBM using red light LED may induce proliferation and progression of HT29 cancer cells by increasing mitochondrial activity and fission.Copyright © 2024 Elsevier B.V. All rights reserved.