组织库程序不断发展，以跟上精准医学、技术、对种族差异的新认识和监管要求的步伐。然而，关于创建和维护成功的生物样本库的策略，几乎没有公布的指南。我们的目标是描述我们的妇科肿瘤组织库使用的基础设施和协议。我们的组织库成立于 1992 年。2022 年 8 月，内部资金用于对组织库进行现代化改造。我们雇用了三名全职员工，对妇科肿瘤学人员治疗的患者进行了普遍筛查，更新了同意协议，并标准化了与提供者的沟通。从符合条件且同意的患者身上收集肿瘤组织、血液衍生物、腹水和胸腔积液并进行处理。产生了源自患者的细胞系和类器官。出于质量控制目的，由委员会认证的病理学家对每个组织部位一份福尔马林固定、石蜡包埋 (FFPE) 样本进行分析。所有样本均按照 OpenSpecimen 采集方案进行标记和跟踪，并在安全数据库中进行临床注释。从 2022 年 8 月至 2023 年 10 月，招募了 227 名患者（83% 白人、15% 黑人、1% 亚洲人），收集了 4249 份样本。从 15 名卵巢癌患者中产生贴壁细胞系，并从 46 名卵巢癌患者中收集用于类器官生成的细胞悬液。建立了一个补充中心来自我维持组织库。样本已与学术和商业合作者共享。我们的组织库招募了大量不同的患者，收集了多种样本类型，并进行了广泛的合作。此处描述的程序为其他机构建立类似资源提供了指导。版权所有 © 2024。由 Elsevier Inc. 出版。

Tissue banking procedures have evolved to keep pace with precision medicine, technology, emerging understanding of racial disparities, and regulatory requirements. However, there is little published guidance regarding strategies to create and maintain a successful biorepository. Our objective is to describe the infrastructure and protocols used by our Gynecologic Oncology Tissue Bank.Our Tissue Bank was founded in 1992. In August 2022, internal funding was used to modernize the Tissue Bank. We hired three full-time employees, implemented universal screening of patients treated by gynecologic oncology faculty, updated consenting protocols, and standardized communication with providers. Tumor tissue, blood derivatives, ascites, and pleural fluid were collected from eligible, consenting patients and processed. Patient-derived cell lines and organoids were generated. For quality control purposes, one formalin-fixed, paraffin-embedded (FFPE) sample per tissue site was analyzed by a board-certified pathologist. All samples were labeled and tracked in an OpenSpecimen collection protocol and clinically annotated in a secure database.From August 2022 to October 2023, 227 patients (83% white, 15% Black, 1% Asian) were enrolled and 4249 specimens were collected. Adherent cell lines were generated from 15 patients with ovarian cancer and cell suspensions for organoid generation were collected from 46 patients with ovarian cancer. A recharge center was established to self-sustain the Tissue Bank. Samples have been shared with academic and commercial collaborators.Our Tissue Bank has enrolled a large number of diverse patients, collected numerous specimen types, and collaborated widely. The procedures described here provide guidance for other institutions establishing similar resources.Copyright © 2024. Published by Elsevier Inc.