提出了一种结合光电化学（PEC）和电化学（EC）的生物功能免疫传感器，用于定量检测人血液中肝癌标志物甲胎蛋白（AFP）。首先在导电玻璃FTO上制备BiVO4/BiOI-MWCNTs光活性材料，并通过壳聚糖和戊二醛对光电极进行功能化。然后，在光电极上成功修饰AFP捕获抗体（Ab1），并通过PEC实现了AFP抗原的无标记快速检测。此外，Au@PdPt纳米球也被用作与AFP检测抗体（Ab2）结合的标记物。由于Au@PdPt在EC反应中具有优异的催化特性，当Ab2与AFP抗原结合时，可以实现EC反应的信号增强，从而保证了AFP检测的高灵敏度。 PEC 和 EC 的检测限分别为 0.050 pg/mL 和 0.014 pg/mL。该传感器还具有良好的特异性、稳定性和重现性，在临床样本的检测中表现出优异的性能，具有良好的临床适用性。Copyright © 2024. Published by Elsevier B.V.

A biofunctional immunosensor combining photoelectrochemical (PEC) and electrochemical (EC) was proposed for the quantitative detection of the liver cancer marker alpha-fetoprotein (AFP) in human blood. BiVO4/BiOI-MWCNTs photoactive materials were first prepared on conductive glass FTO, and the photoelectrode was functionalized by chitosan and glutaraldehyde. Then, the AFP capture antibody (Ab1) was successfully modified on the photoelectrode, and the label-free rapid detection of AFP antigen was achieved by PEC. In addition, Au@PdPt nanospheres were also used as a marker for binding to AFP detection antibody (Ab2). Due to the excellent catalytic properties of Au@PdPt in EC reaction, a signal increase in the EC response can be achieved when Ab2 binds to the AFP antigen, which ensures high sensitivity for the detection of AFP. The detection limits of PEC and EC are 0.050 pg/mL and 0.014 pg/mL, respectively. The sensor also possesses good specificity, stability and reproducibility, shows excellent performance in the detection of clinical samples and has good clinical applicability.Copyright © 2024. Published by Elsevier B.V.