本文描述了基于刺槐豆胶稳定的纳米金并用叶下珠花青素功能化的抗癌纳米平台（LBG/PRA-NG）的设计和制造。 LBG/PRA-NG 是在室温下以环保的一锅法制备的，分别由花青素和树胶作为生物还原剂和稳定剂介导。通过 FESEM、TEM、UV-visible、DLS、Zeta 电位、FTIR、XRD、TGA/DTG 和 XPS 分析对纳米结构进行了详细表征。根据对 MCF-7 和 MDA-MB-231 乳腺癌细胞系的细胞毒性以及细胞内活性氧的产生来检查其抗癌特性。结果表明，成功形成了均匀且高度稳定的纳米复合材料（LBG/PRA-NG），其形状呈准球形，尺寸小（14.73nm），Zeta电位和PDI值分别为-58.30mV和0.237。 525 nm 处等离子体峰的存在表明存在 AuNP。与半乳甘露聚糖和花青素相比，LBG/PRA-NG表现出优异的抗氧化性能，对DPPH的IC50值为35.44μg/mL，对ABTS的IC50值为24.55μg/mL。值得注意的是，LBG/PRA-NG 还表现出相对于 LBG 和花青素增强的抗癌特性，对 MCF-7 和 MDA-MB-231 细胞的 IC50 值为 16.17μg/mL 和 25.06μg/mL。同时，正常细胞（HEK-293和L929）抵抗LBG/PRA-NG的无害作用。此外，用 LBG/PRA-NG 处理乳腺癌细胞可显着提高细胞内 ROS 水平。这表明LBG/PRA-NG的抗癌活性可能是通过ROS/氧化应激诱导的细胞凋亡的放大来介导的。总而言之，这些发现表明了 LBG/PRA-NC 在抗癌治疗发展中的巨大潜力。版权所有 © 2024。由 Elsevier B.V. 出版。

Herein, the design and fabrication of an anticancer nanoplatform (LBG/PRA-NG) based on locust bean gum-stabilized nanogold and functionalized with Phyllanthus reticulatus anthocyanins was described. LBG/PRA-NG was prepared in an eco-friendly, one-pot approach at room temperature, mediated by the anthocyanins and gum as bio-reductant and stabilizer, respectively. The nanostructure was elaborately characterized by FESEM, TEM, UV-visible, DLS, Zeta potential, FTIR, XRD, TGA/DTG, and XPS analysis. Its anticancer attributes were examined based on cytotoxicity on MCF-7 and MDA-MB-231 breast cancer cell lines, as well as the generation of intracellular reactive oxygen species. The results revealed the successful formation of a homogenous and highly stable nanocomposite (LBG/PRA-NG), with quasi-spherical shape, small size (14.73 nm), Zeta potential and PDI values of -58.30 mV and 0.237, respectively. The presence of a plasmonic peak at 525 nm was indicative of AuNPs. Compared to the galactomannan and anthocyanin, LBG/PRA-NG exhibited superior antioxidative properties with IC50 values of 35.44 μg/mL against DPPH and 24.55 μg/mL against ABTS+. Notably, LBG/PRA-NG also demonstrated enhanced anticancer properties relative to LBG and anthocyanins, with IC50 values of 16.17 μg/mL and 25.06 μg/mL against MCF-7 and MDA-MB-231 cells. Meanwhile, the normal cells (HEK-293 and L929) resisted the innocuous effects of LBG/PRA-NG. Furthermore, treatment of breast cancer cells with LBG/PRA-NG drastically elevated the intracellular ROS levels. This suggested that the anticancer activity of LBG/PRA-NG may be mediated via amplification of ROS/oxidative stress-induced apoptosis. Altogether, these findings indicate the remarkable potential of LBG/PRA-NC in the development of anticancer therapy.Copyright © 2024. Published by Elsevier B.V.