胚胎植入是成功怀孕的第一步。胚胎植入的体外模型对于基础生物学研究、药物开发和筛选至关重要。本文提出了一种简单、快速、高效的胚胎植入体外模型。在本协议中，我们首先介绍小鼠囊胚的获取和人子宫内膜腺癌细胞（石川）植入的准备，然后介绍小鼠胚胎和石川细胞的共培养方法。最后，我们基于该模型进行了一项研究，评估不同浓度的17-β-雌二醇（E2）和黄体酮（P4）对胚胎粘附率的影响。我们的研究结果表明，高浓度的 E2 显着降低了胚胎粘附，而添加黄体酮则可以恢复粘附率。该模型提供了一个简单快速的平台，用于评估和筛选参与粘附过程的分子，例如控制着床和子宫内膜容受性的细胞因子、药物和转录因子。

Embryo implantation is the first step in the establishment of a successful pregnancy. An in vitro model for embryo implantation is critical for basic biological research, drug development, and screening. This paper presents a simple, rapid, and highly efficient in vitro model for embryo implantation. In this protocol, we first introduce mouse blastocyst acquisition and human endometrial adenocarcinoma cells (Ishikawa) preparation for implantation, followed by the co-culture method for mouse embryos and Ishikawa cells. Finally, we conducted a study to assess the impact of varying concentrations of 17-β-estradiol (E2) and progesterone (P4) on embryo adhesion rates based on this model. Our findings revealed that high concentrations of E2 significantly reduced embryo adhesion, whereas the addition of progesterone could restore the adhesion rate. This model offers a simple and fast platform for evaluating and screening molecules involved in the adhesion process, such as cytokines, drugs, and transcription factors controlling implantation and endometrial receptivity.