某些免疫细胞的动员可能会提高免疫系统对抗肿瘤细胞的能力，但急性运动对动员免疫细胞的影响在癌症患者中的研究很少。因此，我们研究了剧烈运动如何影响乳腺癌患者的循环免疫细胞。 19 名年龄在 36-68 岁之间的新诊断乳腺癌患者使用自行车测力计进行了 30 分钟的中等强度运动。在不同时间点采集血样：休息时、运动开始后 15 分钟 (E15) 和 30 分钟 (E30)、运动后 30 分钟和 60 分钟。我们使用流式细胞术分析了几种免疫细胞亚群。急性运动增加了白细胞总数、中性粒细胞、淋巴细胞、单核细胞、嗜碱性粒细胞、T 细胞总数、CD4 T 细胞、辅助性 T (Th) 2 细胞、Th 17 细胞的数量、CD8 T 细胞、CD4-CD8- T 细胞、CD56 自然杀伤 (NK) 细胞和 CD14-CD16 单核细胞。许多变化都是短暂的。通过运动，NK 细胞和 CD8 T 细胞的比例增加，而骨髓源性抑制细胞 (MDSC) 的比例减少，调节性 T 细胞的比例保持不变。在细胞动员和疾病状态之间发现了多种关联。例如，E15时肿瘤大小与NK细胞动员呈负相关，孕激素受体阳性与CD8 T细胞动员呈负相关。研究结果表明，E15时CD8 T细胞和NK细胞的比例增加，MDSC比例下降。乳腺癌患者经过 30 分钟的运动后，表明循环免疫细胞的特征发生了变化，变得更具细胞毒性/抗肿瘤发生作用。一些免疫细胞的动员似乎也与疾病状态有关。版权所有 © 2024 Koivula、Lempiäinen、Neuvonen、Norha、Hollmén、Sundberg、Rundqvist、Minn、Rinne 和 Heinonen。

Mobilization of certain immune cells may improve the ability of the immune system to combat tumor cells, but the effect of acute exercise on mobilizing immune cells has been sparsely investigated in cancer patients. Therefore, we examined how acute exercise influences circulating immune cells in breast cancer patients.Nineteen newly diagnosed breast cancer patients aged 36-68 performed 30 minutes of moderate-intensity exercise with a cycle ergometer. Blood samples were collected at various time points: at rest, at 15 (E15) and 30 minutes (E30) after onset of the exercise, and at 30 and 60 minutes post-exercise. We analyzed several immune cell subsets using flow cytometry.Acute exercise increased the number of total leukocytes, neutrophils, lymphocytes, monocytes, basophils, total T-cells, CD4+ T-cells, T helper (Th) 2-cells, Th 17-cells, CD8+ T-cells, CD4-CD8- T-cells, CD56+ natural killer (NK) cells, and CD14-CD16+ monocytes. Many of the changes were transient. Proportions of NK-cells and CD8+ T-cells increased, while the proportion of myeloid derived suppressor cells (MDSCs) reduced, and proportion of regulatory T-cells remained unchanged by exercise. Several associations were detected between cell mobilizations and disease state. For instance, tumor size correlated negatively with NK cell mobilization at E15, and progesterone receptor positivity correlated negatively with CD8+ T-cell mobilization.The findings show that the proportions of CD8+ T-cells and NK cells increased and the proportion of MDSCs proportion decreased in breast cancer patients after 30-minute exercise, suggesting a change in the profile of circulating immune cells towards more cytotoxic/anti-tumorigenic. The mobilization of some immune cells also appears to be related to the disease state.Copyright © 2024 Koivula, Lempiäinen, Neuvonen, Norha, Hollmén, Sundberg, Rundqvist, Minn, Rinne and Heinonen.