荧光共焦显微镜 (FCM) 是一种光学技术，使用不同波长的激光光源生成新鲜、未固定组织标本的实时图像。 FCM 可以对新鲜组织样本进行组织学评估，而在冷冻切片后不会产生相关的冷冻伪影。本研究的目的是前瞻性评估儿科肿瘤标本并评估其对新鲜肿瘤取样的适用性。此外，我们的目的是确定在 FCM 成像后，稳定细胞培养的肿瘤细胞分离是否仍然可行。使用 FCM 对儿科肿瘤标本进行成像。评估肿瘤活力和组织取样的适用性，并与 H 进行比较

Fluorescence confocal microscopy (FCM) is an optical technique that uses laser light sources of different wavelengths to generate real-time images of fresh, unfixed tissue specimens. FCM allows histological evaluation of fresh tissue samples without the associated cryo artifacts after frozen sectioning. The aim of this study was to prospectively evaluate pediatric tumor specimens and assess their suitability for fresh tumor sampling. In addition, we aimed to determine whether tumor cell isolation for stable cell culture is still feasible after FCM imaging. Pediatric tumor specimens were imaged using FCM. Tumor viability and suitability for tissue sampling were evaluated and compared with H&E staining after paraffin embedding. In addition, FCM-processed and non-FCM-processed tissue samples were sent for tumor cell isolation to evaluate possible effects after FCM processing. When comparing estimated tumor cell viability using FCM and H&E, we found good to excellent correlating estimates (intraclass correlation coefficient = 0.891, p < 0.001), as well as substantial agreement in whether the tissue appeared adequate for fresh tissue collection (κ = 0.762, p < 0.001). After FCM, seven out of eight samples yielded passable cell cultures, compared to eight out of eight for non-FCM processed samples. Our study suggests that the use of FCM in tumor sampling can increase the yield of suitable fresh tumor samples by identifying viable tumor areas and ensuring that sufficient tissue remains for diagnosis. Our study also provides first evidence that the isolation and growth of tumor cells in culture are not compromised by the FCM technique.© 2024. The Author(s).