使用Malus sylvestris L. (LE1)、Pinus sylvestris L. (LE2)和Sorbus aucuparia L. (LE3)叶子提取物合成含有不同量氯化银(AgCl)、银的银(Ag)纳米复合材料。金属（Agmet）和磷酸银（Ag3PO4）。这些纳米复合材料被叶子提取物中的有机官能团封端。值得注意的是，纳米复合材料引起了细胞的双相细胞毒性反应。首先归因于细胞生长的抑制，其次归因于细胞死亡。纳米复合材料与正常胚胎肾（HEK293）细胞在癌细胞的细胞毒性范围内具有生物相容性。 LE2Ag1 [25(±1) °C 合成]纳米复合材料在孵育 24 小时后对 HeLa（致死浓度 - LC50 值为 11.4μgmL-1）和 A549（LC50 值为 14.7μgmL-1）表现出最高的细胞毒性及其耐药性更强的 MCF-7 和 MDA-MB-231 也显示出效率，但它们各自的 LC50 值更大。对于HeLa细胞系，这种设计的LE2Ag1纳米复合材料表现出与顺铂的有效浓度（EC50）值相似的LC50值，并且比阿霉素大约3倍。与所有其他纳米复合材料相比，LE2Ag1 纳米复合材料复合材料中含有 Ag3PO4，表面含有 P，AgCl 含量更高，所有纳米颗粒相的晶粒尺寸更小，并且表面富碳缺氧。版权所有 © 2024。由 Elsevier B.V. 出版。

The Malus sylvestris L. (LE1), Pinus sylvestris L. (LE2), and Sorbus aucuparia L. (LE3) leaves` extracts were used for the synthesis of silver (Ag) nanocomposites containing different amounts of silver chloride (AgCl), silver metal (Agmet), and silver phosphate (Ag3PO4). These nanocomposites were capped with the organic functional groups in the leaf extract. Notably, the nanocomposites caused biphasic cytotoxic response on cells; first attributed to the inhibition of cell growth and second to cell death. The nanocomposites were biocompatible with normal embryonic kidney (HEK293) cells in the cytotoxic range for cancer cells. LE2Ag1 [25(±1) °C synthesis] nanocomposites exhibited the highest cytotoxicity towards HeLa (lethal concentration- LC50 value of 11.4 μg mL-1) and A549 (LC50 value of 14.7 μg mL-1) after 24-h incubation and its efficiency was shown also for the more resistant MCF-7 and MDA-MB-231, however, their respective LC50 values were larger. For the HeLa cell line, this designed LE2Ag1 nanocomposite exhibited an LC50 value similar to the effective concentration (EC50) value of Cisplatin and about 3 times larger than Doxorubicin. LE2Ag1 nanocomposite contained Ag3PO4 in the composite and P on the surface, higher AgCl content, smaller crystallite size of all nanoparticle phases, and carbon-rich oxygen-deficient surface compared to all other nanocomposites.Copyright © 2024. Published by Elsevier B.V.