DNA甲基化是一种重要的分子修饰，在癌症基因的表达中发挥着关键作用。评估特定基因的表观遗传变化、低甲基化和高甲基化可用于癌症诊断。许多研究集中于描述 DNA 甲基化模式作为癌症诊断监测和预测癌症治疗反应的生物标志物。用于检测癌症中 DNA 甲基化状态的各种技术都是基于亚硫酸氢钠治疗。根据这些方法在研究和临床研究中的应用，它们具有许多优点和缺点。当前的综述重点介绍了基于亚硫酸氢钠治疗的技术，以及这些方法在人类癌症评估中的优点、缺点和应用。© 2024。作者。

DNA methylation is an important molecular modification that plays a key role in the expression of cancer genes. Evaluation of epigenetic changes, hypomethylation and hypermethylation, in specific genes are applied for cancer diagnosis. Numerous studies have concentrated on describing DNA methylation patterns as biomarkers for cancer diagnosis monitoring and predicting response to cancer therapy. Various techniques for detecting DNA methylation status in cancers are based on sodium bisulfite treatment. According to the application of these methods in research and clinical studies, they have a number of advantages and disadvantages. The current review highlights sodium bisulfite treatment-based techniques, as well as, the advantages, drawbacks, and applications of these methods in the evaluation of human cancers.© 2024. The Author(s).