肽功能化磁性纳米颗粒（Pep@MNP）检测系统可以捕获胰腺导管腺癌（PDAC）中的循环肿瘤细胞（CTC）。检测CTC和CXCR4表达，探讨其临床意义。 CXCR4 CTC，这是一种高度转移倾向的干细胞样 CTC 亚群 (HM-CTC)，被发现与 PDAC 的早期复发和转移相关。CTC 被 Pep@MNP 捕获。通过CD45、细胞角蛋白抗体免疫荧光对CTC进行鉴定，CXCR4阳性的CTC被指定为HM-CTC。CXCR4的过表达可以促进胰腺癌细胞的体外和体内迁移。外周血（PB）中，79.0%的患者（49/62，9（0-71）/2mL）检测到CTC阳性，其中63.3%的患者（31/49，3（0-23）/2mL） ) HM-CTC 呈阳性。在门静脉血 (PVB) 中，77.5% 的患者 (31/40, 10 (0-40)/2mL) CTC 呈阳性，其中 67.7% (21/31, 4 (0-15)/2mL) HM-CTCs 阳性 CTCs 计数可作为胰腺癌的诊断生物标志物（AUC = 0.862），CTCs 阳性和 CA19-9 增加的组合显示诊断准确性提高（AUC = 0.963）。此外，PVB HM-CTCs比PB HM-CTCs更准确地预测早期复发和肝转移（AUC分别为0.825 vs. 0.787和0.827 vs. 0.809）。Pep@MNP检测系统识别的CTCs可以使用作为 PDAC 患者的诊断和预后生物标志物。我们鉴定并定义了CXCR4过表达的CTC亚群为高度转移倾向的CTC，这被证明可以识别容易遭受早期复发和转移的患者。版权所有©2024 Chu，Zhong，Zang，Wang，Li，Ma，田、杨、王、杨。

The circulating tumor cells (CTCs) could be captured by the peptide functionalized magnetic nanoparticles (Pep@MNP) detection system in pancreatic ductal adenocarcinoma (PDAC). CTCs and the CXCR4 expression were detected to explore their clinical significance. The CXCR4+ CTCs, this is highly metastatic-prone stem cell-like subsets of CTCs (HM-CTCs), were found to be associated with the early recurrence and metastasis of PDAC.CTCs were captured by Pep@MNP. CTCs were identified via immunofluorescence with CD45, cytokeratin antibodies, and the CXCR4 positive CTCs were assigned to be HM-CTCs.The over-expression of CXCR4 could promote the migration of pancreatic cancer cell in vitro and in vivo. In peripheral blood (PB), CTCs were detected positive in 79.0% of all patients (49/62, 9 (0-71)/2mL), among which 63.3% patients (31/49, 3 (0-23)/2mL) were HM-CTCs positive. In portal vein blood (PVB), CTCs were positive in 77.5% of patients (31/40, 10 (0-40)/2mL), and 67.7% of which (21/31, 4 (0-15)/2mL) were HM-CTCs positive CTCs enumeration could be used as diagnostic biomarker of pancreatic cancer (AUC = 0.862), and the combination of CTCs positive and CA19-9 increase shows improved diagnostic accuracy (AUC = 0.963). in addition, PVB HM-CTCs were more accurate to predict the early recurrence and liver metastasis than PB HM-CTCs (AUC 0.825 vs. 0.787 and 0.827 vs. 0.809, respectively).The CTCs identified by Pep@MNP detection system could be used as diagnostic and prognostic biomarkers of PDAC patients. We identified and defined the CXCR4 over-expressed CTC subpopulation as highly metastatic-prone CTCs, which was proved to identify patients who were prone to suffering from early recurrence and metastasis.Copyright © 2024 Chu, Zhong, Zang, Wang, Li, Ma, Tian, Yang, Wang and Yang.