菠萝蜜叶蛋白水解物是通过胃肠道酶对叶蛋白浓缩物进行酶解而获得的，具有良好的技术功能特性和高抗氧化能力。然而，其分子量、抗增殖活性、细胞毒性和减少活性氧（ROS）的能力仍然未知。因此，本研究旨在评估在不同水解时间（30-240分钟）下用胃蛋白酶和胰酶酶解获得的菠萝蜜叶蛋白水解物对分子量、细胞毒性、抗癌细胞增殖和减少活性氧的影响。在 H2O2 诱导的 HaCaT 细胞中。电泳图谱表明 H-Pep 含有分子量在 25 - 20 kDa 之间的肽。同时，H-Pan 由分子量介于 25 - 20 kDa 和 < 20 kDa 之间的产品组成。 H-Pan 和 H-Pep (125-500 µg/mL) 对 HaCaT（人角质形成细胞）和 J774A.1（鼠巨噬细胞）没有表现出显着的细胞毒性。在人宫颈癌、卵巢癌和肝癌细胞中取得了抗增殖活性。 H-Pan-240 分钟 (1000 µg/mL) 使宫颈癌细胞的细胞活力降低 23%，而 H-Pan-60 分钟则使卵巢癌细胞和肝癌细胞的细胞活力显着降低 14.5% (500 µg/mL) 和 17% % (1000 µg/mL)，分别 (P < 0.05)。 H-Pep-60 分钟对 H2O2 应激的 HaCaT 细胞具有抗氧化应激的保护作用，在 250 µg/mL 浓度下可减少 25% 的 ROS（P<0.05）。研究结果证明了绿色生物质作为植物蛋白水解物来源的安全使用。© 2024。作者获得 Springer Science Business Media, LLC（Springer Nature 旗下公司）的独家许可。

Jackfruit leaf protein hydrolysates obtained from the enzymatic hydrolysis of leaf protein concentrate with gastrointestinal enzymes have shown good techno-functional properties and high antioxidant capacity. However, molecular weight, antiproliferative activity, cytotoxicity and the ability to reduce reactive oxygen species (ROS) are still unknown. Therefore, this study aimed to evaluate the effect of jackfruit leaf protein hydrolysates obtained by enzymatic hydrolysis with pepsin and pancreatin at different hydrolysis times (30-240 min) on molecular weights, cytotoxicity, antiproliferation of cancer cells, and the reduction of reactive oxygen species in H2O2-induced HaCaT cells. The electrophoretic profile indicated that H-Pep contains peptides with molecular weights between 25 - 20 kDa. Meanwhile, H-Pan is composed of molecular weight products between 25 - 20 kDa and < 20 kDa. H-Pan and H-Pep (125-500 µg/mL) did not show significant cytotoxicity on HaCaT (human keratinocytes) and J774A.1 (murine macrophage cells). Antiproliferative activity was achieved in human cervical, ovarian, and liver cancer cells. H-Pan-240 min (1000 µg/mL) reduced the cell viability of cervical cancer cells by 23% while H-Pan-60 min significantly reduced cell viability of ovarian and liver cancer cells by 14.5 (500 µg/mL) and 17% (1000 µg/mL), respectively (P < 0.05). The protective effect against oxidative stress on H2O2-stressed HaCaT cells was obtained with H-Pep-60 min, which reduced 25% of ROS at 250 µg/mL (P < 0.05). The findings demonstrate the safe use of green biomass as a source of plant protein hydrolysates.© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.