过氧化氢 (H2O2) 是一种关键的活性氧 (ROS)，在与细胞增殖、分化、迁移和疾病进展相关的氧化还原信号通路和免疫反应中发挥着至关重要的作用。选择性监测过量产生的 H2O2 对于了解心血管疾病、癌症、糖尿病、帕金森病、阿尔茨海默病和炎症等疾病的诊断和发病机制非常重要。本文通过将硼酸苯酯与荧光团BQM-PNH连接，开发了一种AIE荧光探针BQM-H2O2，用于选择性检测H2O2。在 fw = 99%（pH = 7.4，1% DMSO）存在 H2O2 的情况下，探针 BQM-H2O2 会因硼酸酯的氧化而产生强烈的荧光信号。该探针对 H2O2 表现出高选择性和低检测限，计算出的 LOD 为 112.6 nM。重要的是，它用于检测 4T1 细胞中的外源性和内源性过氧化氢，细胞毒性较低。该探针还成功应用于携带 4T1 移植肿瘤的 Blab/c 小鼠的 H2O2 成像。版权所有 © 2024。由 Elsevier Inc. 出版。

Hydrogen peroxide (H2O2), a key reactive oxygen species (ROS), plays crucial roles in redox signaling pathways and immune responses associated with cell proliferation, differentiation, migration, and disease progression. The selective monitoring of overproduced H2O2 is important for understanding the diagnosis and pathogenesis of diseases such as cardiovascular disease, cancers, diabetes, Parkinson's disease, Alzheimer's disease, and inflammation. In this paper, an AIE fluorescent probe BQM-H2O2 was developed by connecting phenyl borate with the fluorophore BQM-PNH for selective detection of H2O2. In the presence of H2O2 at fw = 99% (pH = 7.4, 1% DMSO), the probe BQM-H2O2 could generate strong fluorescent signals due to the oxidation of the borate ester. The probe exhibited high selectivity and a low detection limit toward H2O2 with the calculated LOD of 112.6 nM. Importantly, it was employed in the detection of exogenous and endogenous hydrogen peroxide in 4T1 cells with low cytotoxicity. This probe has also been successfully applied to imaging of H2O2 in Blab/c mice bearing 4T1 graft tumors.Copyright © 2024. Published by Elsevier Inc.