目前的治疗方案无法治愈高级别神经胶质瘤并预防复发。因此，需要开发新的方法。神经胶质瘤细胞命运的重新编程是阻止肿瘤生长的另一种有吸引力的方法。两步方案应用抗增殖 GQ bi-(AID-1-T) 和小分子诱导剂与 BDNF 来触发神经分化为终末分化细胞，对 GB 细胞培养物非常有效。这种独创的方法是“分化疗法”的成功范例。为了证明这种方法的多功能性，在本出版物中，我们将一系列细胞培养物扩展到 II、III 和 IV 级神经胶质瘤，并证明了该版本的差异化治疗对各种肿瘤细胞的适用性。我们已经证明了向神经胶质瘤细胞添加 GQIcombi 成分的顺序模式的合理性。我们已经显示，在模型 101/8 的大鼠脑部肿瘤中直接注射 GQIcombi 后，肿瘤生长显着延缓。因此，所提出的影响癌细胞生长的策略适用于进一步转化为治疗用途。版权所有 © 2024 Kolesnikova、Revishchin、Fab、Alekseeva、Ryabova、Pronin、Usachev、Kopylov 和 Pavlova。

Current therapy protocols fail to cure high-grade gliomas and prevent recurrence. Therefore, novel approaches need to be developed. A re-programing of glioma cell fate is an alternative attractive way to stop tumor growth. The two-step protocol applies the antiproliferative GQ bi-(AID-1-T) and small molecule inducers with BDNF to trigger neural differentiation into terminally differentiated cells, and it is very effective on GB cell cultures. This original approach is a successful example of the "differentiation therapy". To demonstrate a versatility of this approach, in this publication we have extended a palette of cell cultures to gliomas of II, III and IV Grades, and proved an applicability of that version of differential therapy for a variety of tumor cells. We have justified a sequential mode of adding of GQIcombi components to the glioma cells. We have shown a significant retardation of tumor growth after a direct injection of GQIcombi into the tumor in rat brain, model 101/8. Thus, the proposed strategy of influencing on cancer cell growth is applicable to be further translated for therapy use.Copyright © 2024 Kolesnikova, Revishchin, Fab, Alekseeva, Ryabova, Pronin, Usachev, Kopylov and Pavlova.