乳腺癌是生殖系统最常见的恶性肿瘤之一，预后较差。本研究的目的是探讨突触结合蛋白7（SYT7）在乳腺癌中的功能和潜在机制。我们利用癌症基因组图谱（TCGA）数据库和Kaplan-Meier绘图仪数据库来评估SYT7表达与预后之间的相关性的乳腺癌患者。通过逆转录定量聚合酶链反应（RT-qPCR）和蛋白质印迹评估SYT7敲低的功效。此外，我们使用细胞计数试剂盒-8 (CCK-8)、克隆形成测定和流式细胞术检查了 SYT7 对乳腺癌细胞增殖和凋亡的影响。通过Western blot分析，我们研究了SYT7对乳腺癌组织中凋亡相关标志物表达及PI3K/AKT信号通路的影响。TCGA数据库数据分析显示，与正常对照组相比，乳腺癌组织中SYT7表达显着上调。正常组织（P<0.001）。在乳腺癌患者中观察到SYT7表达与肿瘤大小（P=0.009）、雌激素受体（ER）表达水平（P<0.001）和孕激素受体（PR）表达水平（P<0.001）之间存在相关性。 Kaplan-Meier 绘图仪数据库的分析表明，高 SYT7 表达与较短的总生存期 (OS) 相关 (P=0.009)。 mRNA表达结果显示，乳腺癌组织中SYT7表达量高于癌旁正常组织（P=0.005）。 CCK-8、克隆形成实验和流式细胞术结果表明SYT7促进乳腺癌细胞增殖并抑制乳腺癌细胞凋亡。 Western blot检测证实了SYT7对PI3K/AKT信号的激活。研究结果表明SYT7在乳腺癌中高表达，并且其高表达与临床特征和预后相关。通过敲低抑制SYT7可以抑制乳腺癌细胞的增殖并促进其凋亡，使其成为乳腺癌诊断和治疗的潜在靶点。2024转化癌症研究。版权所有。

Breast cancer is one of the most malignant tumors in the reproductive system and has a poor prognosis. The aim of this study was to investigate the function and underlying mechanism of synaptotagmin 7 (SYT7) in breast cancer.We utilized The Cancer Genome Atlas (TCGA) database and the Kaplan-Meier plotter database to assess the correlation between SYT7 expression and the prognosis of breast cancer patients. The efficacy of SYT7 knockdown was evaluated through reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting. Furthermore, we examined the impact of SYT7 on breast cancer cell proliferation and apoptosis using Cell Counting Kit-8 (CCK-8), clone formation assays, and flow cytometry. Through Western blot analysis, we investigated the influence of SYT7 on the expression of apoptosis-related markers and the PI3K/AKT signaling pathway in breast cancer.The TCGA database data analysis revealed a significant up-regulation of SYT7 expression in breast cancer tissues compared to normal tissues (P<0.001). A correlation was observed between SYT7 expression and tumor size (P=0.009), as well as estrogen receptor (ER) expression level (P<0.001) and progesterone receptor (PR) expression level (P<0.001) in breast cancer patients. Analysis of the Kaplan-Meier plotter database indicated that high SYT7 expression was associated with a shorter overall survival (OS) (P=0.009). The mRNA expression results indicated higher SYT7 expression in breast cancer tissues compared to adjacent normal tissues (P=0.005). CCK-8, clone formation assay, and flow cytometry results demonstrated that SYT7 promoted the proliferation and inhibited the apoptosis of breast cancer cells. Western blot assay confirmed the activation of PI3K/AKT signaling by SYT7.The findings suggest that SYT7 is highly expressed in breast cancer and that its high expression is linked to clinical characteristics and prognosis. Inhibition of SYT7 through knockdown can suppress proliferation and promote apoptosis of breast cancer cells, making it a potential target for breast cancer diagnosis and treatment.2024 Translational Cancer Research. All rights reserved.