华支睾吸虫是一种重要的人畜共患食源性寄生虫，可引起一系列肝胆疾病，严重时甚至可导致胆管癌。为了探索新的诊断和治疗策略，利用全转录组测序分析了中华线虫不同发育阶段的动态RNA调控过程。使用中华线虫的染色体水平基因组进行序列比对和注释。在这项研究中，我们在全基因组中总共鉴定了 59,103 个 RNA，包括 2,384 个 miRNA、25,459 个 mRNA、27,564 个 lncRNA 和 3,696 个 circRNA。差异表达分析鉴定了不同发育阶段的 6,556 个差异表达 mRNA、2,231 个 lncRNA、877 个 miRNA 和 20 个 circRNA。功能富集分析强调了微管相关生物过程在中华线虫生长和发育中的关键作用。共表达分析揭示了97个lncRNA和85个circRNA与42个差异表达的mRNA共表达，这些差异表达的mRNA与中华蠹不同发育阶段的微管相关。微管相关基因动力蛋白轻链2（DLC2）和动力蛋白轻链4（DLC4）的表达随着中华线虫的发育而增加，并且DLC2/4可以被阿苯达唑抑制。最后，通过构建竞争性内源RNA（ceRNA）网络，构建了lncRNA-miRNA-mRNA和circRNA-miRNA-mRNA调控关系，并构建了MSTRG.14258.5-novel_miR_2287-newGene_28215和MSTRG.14258.5-novel_miR_2216-CSKR_109340的ceRNA网络已验证。这项研究通过全转录组测序表明，微管调控的背景可能在中华线虫的发育和生长中发挥着重要作用。版权所有：© 2024 Qiu et al.这是一篇根据知识共享署名许可条款分发的开放获取文章，允许在任何媒体上不受限制地使用、分发和复制，前提是注明原始作者和来源。

Clonorchis sinensis is a significant zoonotic food-borne parasite that causes a range of hepatobiliary diseases, which in severe cases can even lead to cholangiocarcinoma. To explore new diagnostic and treatment strategies, the dynamic RNA regulatory processes across different developmental stages of C. sinensis were analyzed by using whole-transcriptome sequencing. The chromosomal-level genome of C. sinensis was used for sequence alignment and annotation. In this study, we identified a total of 59,103 RNAs in the whole genome, including 2,384 miRNAs, 25,459 mRNAs, 27,564 lncRNAs and 3,696 circRNAs. Differential expression analysis identified 6,556 differentially expressed mRNAs, 2,231 lncRNAs, 877 miRNAs and 20 circRNAs at different developmental stages. Functional enrichment analysis highlighted the critical role of microtubule-related biological processes in the growth and development of C. sinensis. And coexpression analysis revealed 97 lncRNAs and 85 circRNAs that were coexpressed with 42 differentially expressed mRNAs that associated with microtubules at different developmental stages of C. sinensis. The expression of the microtubule-related genes dynein light chain 2 (DLC2) and dynein light chain 4 (DLC4) increased with C. sinensis development, and DLC2/4 could be inhibited by albendazole. Finally, by constructing competing endogenous RNA (ceRNA) networks, the lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA regulatory relationships were constructed, and the ceRNA networks of MSTRG.14258.5-novel_miR_2287-newGene_28215 and MSTRG.14258.5-novel_miR_2216-CSKR_109340 were verified. This study suggests, through whole transcriptome sequencing, that the context of microtubule regulation may play an essential role in the development and growth of C. sinensis.Copyright: © 2024 Qiu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.