八宝丹（BBD）是中药中独特的中药。可排除毒素、利尿、祛黄。 BBD除了治疗急慢性病毒性肝炎、胆囊炎、胆管炎和尿路感染外，还作为多种恶性肿瘤，特别是肝细胞癌（HCC）的替代治疗而受到欢迎。 阐明BBD单用和联合用药的疗效和机制与卡瑞利珠单抗（CLM）一起治疗HCC。我们研究了BBD对体内HCC肿瘤微环境的影响。此外，我们在体外评估了它对 M2 巨噬细胞诱导的肿瘤生长和转移的影响。在原位 HCC 小鼠模型中，BBD 降低了肿瘤生长。此外，它还增加了小鼠中 M1/M2 巨噬细胞的比率和 CD8 T 细胞丰度。此外，BBD逆转M2巨噬细胞诱导的HCC细胞增殖和转移，增强低剂量CLM的抗HCC作用，并减轻高剂量CLM诱导的器官损伤。最后，BBD通过PI3K/AKT/mTOR信号通路增强CLM的功效。BBD通过调节肿瘤免疫微环境并减弱CLM的毒副作用来增强CLM的抗肿瘤作用。版权所有©2024。由Elsevier B.V.出版。

Babaodan (BBD) is a unique Chinese medication utilized in traditional Chinese medicine. It can eliminate toxins, induce diuresis, and eliminate yellowish hue. In addition to treating acute and chronic viral hepatitis, cholecystitis, cholangitis, and urinary tract infections, BBD has garnered popularity as a substitution treatment for several malignant cancers, particularly hepatocellular carcinoma (HCC).To elucidate the efficacy and mechanism of BBD alone and combined with camrelizumab (CLM) for treating HCC.We investigated the effects of BBD on the HCC tumor microenvironment in vivo. Furthermore, we evaluated its effects on tumor growth and metastasis induced by M2 macrophages in vitro.In a mouse model of orthotopic HCC, BBD decreased tumor growth. Furthermore, it increased the M1/M2 macrophage ratio and CD8+ T-cell abundance in mice. In addition, BBD reversed HCC cell proliferation and metastasis induced by M2 macrophages, increased the anti-HCC effect of low-dose CLM, and attenuated organ damage induced by high-dose CLM. Lastly, BBD enhanced the efficacy of CLM via the PI3K/AKT/mTOR signaling pathway.BBD increases the antitumor effect of CLM by modulating the tumor immune microenvironment and attenuating its the toxic side effects of CLM.Copyright © 2024. Published by Elsevier B.V.