黄芪(AM)及其活性成分主要用于抗炎、抗病毒、抗氧化、免疫调节、心血管和神经系统保护、抗癌、抗肿瘤等。探讨黄芪提取物的药理作用建立右旋糖酐硫酸钠(DSS)诱导的C57BL/6小鼠UC模型，并用黄芪提取物或柳氮磺胺吡啶(SASP)治疗小鼠。 DSS 诱导的小鼠和人源结肠上皮细胞系用于揭示 UC 的炎症环境。黄芪提取物处理后，采用实时定量PCR（qRT-PCR）检测细胞中磷脂酶C-β2（PLCB2）的表达，采用细胞计数试剂盒8（CCK- 8) 测定。最后通过ELISA检测细胞培养上清液中细胞焦亡相关炎症因子的水平。黄芪提取物治疗UC小鼠可显着改善DAI评分和组织病理学损伤评分，并降低Eotaxin、GCSF、KC、MCP水平-1、TNF-α 和 IL-6。此外，黄芪提取物抑制结肠组织中核苷酸结合寡聚化片段样受体家族3（NLRP3）、cleaved Caspase-1和GSDMD-N的表达，并降低炎症相关因子IL-1β的水平血清和组织中的IL-18。在体外，黄芪提取物部分逆转了 DSS 诱导的 CP-M030 和 NCM460 中 PLCB2 表达的降低，促进细胞增殖活性，并降低 IL-1β 和 IL-18 的水平。蒙古蓟提取物通过促进 PLCB2 抑制结肠上皮细胞焦亡来改善溃疡性结肠炎。版权所有 © 2024。由 Elsevier B.V. 出版。

Astragalus mongholicus Bunge (AM) and its active ingredients are mainly used for anti-inflammatory, antiviral, antioxidant, immune regulation, cardiovascular and nervous system protection, anti-cancer, anti-tumor and so on.To explore the Astragalus mongholicus Bunge extract pharmacological mechanisms and biology processes which improves ulcerative colitis (UC).Dextran sulfate sodium (DSS)-induced UC models in C57BL/6 mice were established, and the mice were treated with Astragalus mongholicus Bunge extract or salazosulfapyridine (SASP). DSS-induced mice- and human-derived colonic epithelial cell lines were used to reveal the inflammatory environment of UC. After treatment with Astragalus mongholicus Bunge extract, the expression of phospholipase C-β 2 (PLCB2) in the cells was detected by quantitative real-time PCR (qRT-PCR), and cell proliferative activity was detected by cell counting kit 8 (CCK-8) assay. Finally, the levels of pyroptosis-related inflammatory factors in cell culture supernatants was detected by ELISA.Treatment of UC mice with Astragalus mongholicus Bunge extract do significantly improved DAI scores and histopathological damage scores, and decreased the levels of Eotaxin, GCSF, KC, MCP-1, TNF-α, and IL-6. Besides, Astragalus mongholicus Bunge extract inhibited the expression of nucleotide-binding oligomerization segment-like receptor family 3 (NLRP3), cleaved Caspase-1, and GSDMD-N in the colonic tissues, and reduced the levels of inflammation-related factors IL-1β and IL-18 in serum and tissues. In vitro, Astragalus mongholicus Bunge extract partially reversed the DSS-induced reduction of PLCB2 expression in CP-M030 and NCM460, promoted cell proliferative activity, and reduced the levels of IL-1β and IL-18.In DDS-induced UC mice, Astragalus mongholicus Bunge extract improves ulcerative colitis by inhibiting colonic epithelial cell pyroptosis through PLCB2 promotion.Copyright © 2024. Published by Elsevier B.V.